miR-146a is modulated in human endothelial cell with aging
miR-146a is modulated in human endothelial cell with aging
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DOI：
10.1016/j.atherosclerosis.2011.03.034
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发表时间：
2011-08-01
期刊：
影响因子：
5.3
通讯作者：
Federici, Massimo
中科院分区：
文献类型：
--
作者：
Vasa-Nicotera, Mariuca;Chen, Hailan;Federici, Massimo
背景：越来越多的证据表明，血管内皮细胞的衰老在动脉粥样硬化和血栓形成等血管功能障碍的发病机制中发挥着关键作用。 MicroRNA (miR) 是一种小型非编码 RNA，可通过与其靶 mRNA 的 3'UTR 中的互补序列结合来抑制基因表达。 MiR 调节多种生物功能，例如细胞发育、细胞分化和细胞凋亡。此外，还鉴定了几种参与内皮细胞功能的miR。 方法和结果：通过微阵列方法，我们鉴定了一种随着衰老在内皮细胞中逐渐受到调节的miR-146a。在年轻的人脐静脉内皮细胞中，这种 miR 通过直接靶向 NOX4 蛋白参与早衰样表型，与细胞衰老和老化有关。结论和一般意义：寻找调节内皮细胞衰老的重要因子（如 miR-146a）将有助于为血管疾病提供新的治疗策略。 (C) 2011 Elsevier Ireland Ltd. 保留所有权利。
Background: Increasing evidence has demonstrated that the senescence of vascular endothelial cells has critical roles in the pathogenesis of vascular dysfunction such as atherosclerosis and thrombosis. MicroRNA (miR) are small non-coding RNAs that inhibit gene expression by binding to complementary sequences in the 3'UTR of their target mRNAs. MiRs modulate a variety of biological functions such as cell development, cell differentiation, and apoptosis. Moreover, several miRs involved in endothelial cell function have been identified.Methods and results: Through a microarray approach, we have identified a miR-146a that is progressively modulated in endothelial cells with aging. In young human umbilical vein endothelial cells, this miR is involved in a premature senescence-like phenotype through direct targeting of the NOX4 protein, implicated in cell senescence and aging.Conclusions and general significance: Finding important factors that regulate endothelial cell senescence, like miR-146a, will help provide novel therapeutic strategies for vascular disorders. (C) 2011 Elsevier Ireland Ltd. All rights reserved.