Nanostraws for Direct Fluidic Intracellular Access
Nanostraws for Direct Fluidic Intracellular Access
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DOI：
10.1021/nl204051v
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发表时间：
2012-08-01
期刊：
影响因子：
10.8
通讯作者：
Melosh, Nicholas A.
中科院分区：
文献类型：
--
作者：
VanDersarl, Jules J.;Xu, Alexander M.;Melosh, Nicholas A.
纳米材料是提高 DNA 或药物等活性剂直接进入细胞的递送效率和控制的有前途的候选材料。在这里，我们展示了纳米模板“纳米管”的细胞培养平台，它刺穿细胞膜，提供进入细胞的永久流体管道，以便直接进入细胞质。传统的聚合物径迹蚀刻细胞培养膜经过氧化铝涂层和蚀刻，以产生具有可控直径、厚度和高度的纳米管区域。小分子和离子分别以 40% 和 70% 的效率成功转运到胞质溶胶中，同时 GFP 质粒也成功递送和表达。这些平台为将多种物质主动、可重复地输送到细胞中而无需内吞作用开辟了道路。
Nanomaterials are promising candidates to improve the delivery efficiency and control of active agents such as DNA or drugs directly into cells. Here we demonstrate cell-culture platforms of nanotemplated "nanostraws" that pierce the cell membrane, providing a permanent fluidic pipeline into the cell for direct cytosolic access. Conventional polymeric track-etch cell culture membranes are alumina coated and etched to produce fields of nanostraws with controllable diameter, thickness, and height. Small molecules and ions were successfully transported into the cytosol with 40 and 70% efficiency, respectively, while GFP plasmids were successfully delivered and expressed. These platforms open the way for active, reproducible delivery of a wide variety of species into cells without endocytosis.