The development of non-toxic ionic-crosslinked chitosan-based microspheres as carriers for the controlled release of silk sericin
The development of non-toxic ionic-crosslinked chitosan-based microspheres as carriers for the controlled release of silk sericin
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DOI：
10.1007/s10544-015-9991-4
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发表时间：
2015-10-01
影响因子：
2.8
通讯作者：
Yamdech, Rungnapha
中科院分区：
文献类型：
--
作者：
Aramwit, Pornanong;Ekasit, Sanong;Yamdech, Rungnapha
近年来，丝胶被发现具有多种生物活性，可用于生物医学。虽然各种丝胶载体被开发用于药物传递系统，但很少有研究将丝胶本身作为一种生物活性分子。本研究将丝胶引入壳聚糖基微球中，同时作为生物活性分子和生物活性载体。以无水聚磷酸(TPP)为多阴离子交联剂，制备了不同组成(80/20、70/30、60/40、50/50)的壳聚糖/丝胶(CH/SS)微球。制得的微球平均尺寸为1~4微米，粒径分布较窄。从FT-IR光谱来看，壳聚糖和丝胶在微球中的存在证实了微球中发生了离子相互作用，使它们在微球中相互作用。我们还发现，以50/50的比例制备的CH/SS微球对丝胶的包封率最高(37.28%)，并以最持久的行为释放丝胶，这可能是由于带正电的壳聚糖和带负电的丝胶之间存在强烈的离子相互作用所致。而CH/SS的组成对微球的降解率没有影响。所有微球均持续降解，14d后仍保持在20%左右。这说明壳聚糖和丝胶之间的离子交联可以被酶和丝胶破坏。此外，我们还验证了所有CH/SS微球在任何浓度下对L929小鼠成纤维细胞均无毒性。因此，我们建议将无毒的离子交联型CH/SS微球引入创面敷料材料中，实现丝胶的缓释，加速创面愈合。
Silk sericin is recently shown to possess various biological activities for biomedical applications. While various sericin carriers were developed for drug delivery system, very few researches considered sericin as a bioactive molecule itself. In this study, sericin incorporated in the chitosan-based microspheres was introduced as a bioactive molecule and bioactive carrier at the same time. The chitosan/sericin (CH/SS) microspheres at different composition (80/20, 70/30, 60/40, and 50/50) were successfully fabricated using anhydroustripolyphosphate (TPP) as a polyanionic crosslinker. The microspheres with an average size of 1-4 mu m and narrow size distribution were obtained. From FT-IR spectra, the presence of both chitosan and sericin in the microspheres confirmed the occurrence of ionic interaction that crosslink them within the microspheres. We also found that the CH/SS microspheres prepared at 50/50 could encapsulate sericin at the highest percentage (37.28 %) and release sericin in the most sustained behavior, possibly due to the strong ionic interaction of the positively charged chitosan and the negatively charged sericin. On the other hand, the composition of CH/SS had no effect on the degradation rate of microspheres. All microspheres continuously degraded and remained around 20 % after 14 days of enzymatic degradation. This explained that the ionic crosslinkings between chitosan and sericin could be demolished by the enzyme and hydrolysis. Furthermore, we have verified that all CH/SS microspheres at any concentrations showed non-toxicity to L929 mouse fibroblast cells. Therefore, we suggested that the non-toxic ionic-crosslinked CH/SS microspheres could be incorporated in wound dressing material to achieve the sustained release of sericin for accelerated wound healing.