Characterization and cytokine stimulating activities of heteroglycans from Tremella fuciformis
Characterization and cytokine stimulating activities of heteroglycans from Tremella fuciformis
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DOI：
10.1055/s-2006-957888
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发表时间：
1996-08-01
期刊：
影响因子：
2.7
通讯作者：
Seljelid, R
中科院分区：
文献类型：
--
作者：
Gao, QP;Jiang, RZ;Seljelid, R
从银耳体内分离得到3个杂多糖T1a、T1b和T1c。它们由甘露糖(Man)、木糖(Xyl)、葡萄糖(GLC)、岩藻糖(Fuc)和葡萄糖醛酸(Glca)组成。根据甲基化分析和部分酸性水解，T1a、T1b和T1c的主链由(1->；3)连接的Man组成，在2、4或6位支化。支化点与非还原末端的谷氨酸残基或(1->；6)连接的葡聚糖链相连。这三种杂多糖的相对分子质量分别为53,000，18,000和12,000 D，但它们在水中发生自我聚集。T1a-T1c体外诱导人单核细胞产生白介素1(IL-1)、白介素6(IL-6)和肿瘤坏死因子(TNF)。T1a的酸性水解物部分(T1a-1、2、3、4、5)的相对分子质量从53,000到1,000 D，也可以诱导人单核细胞产生与T1a一样有效的IL-6。
Three heteroglycans, T1a, T1b, and T1c, have been isolated from the body of Tremella fuciformis Berk. They are composed of mannose (Man), xylose (Xyl), glucose (Glc), fucose (Fuc), and glucuronic acid (GlcA). According to methylation analysis and partial acidic hydrolysis the main chains of T1a, T1b, and T1c consisted of (1-->3)-linked Man, which was branched at the 2, 4, or 6 positions. The branching points were linked with nonreducing terminal GlcA-residues or (1-->6)-linked glucan-chains. Molecular weights of the three heteroglycans are 53,000, 18,000, and 12,000 D respectively, but they undergo self-aggregation in water. T1a-T1c induce human monocytes to produce interleukin-1 (IL-1), interleukin-6 (IL-6), and tumor necrosis factor (TNF) in vitro. Acidic hydrolysate fractions of T1a (T1a-1, 2, 3, 4, 5) with a molecular weight from 53,000 to 1,000 D, also induce human monocytes to produce IL-6 as efficient as T1a.