Metaphit amplifies long-term potentiation (LTP) in the mouse hippocampus.
Metaphit amplifies long-term potentiation (LTP) in the mouse hippocampus.
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Metaphit 可增强小鼠海马体的长时程增强 (LTP)。
DOI：
10.1016/0024-3205(94)00905-8
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发表时间：
1995
期刊：
影响因子：
6.1
通讯作者：
Wieraszko,A
中科院分区：
文献类型：
--
作者：
Albanese,R;Hogan,MV;Reith,M;Wieraszko,A
研究了苯环利定衍生物metaphit对小鼠海马长时程增强（LTP）幅度的影响。用metaphit（80 mg/kg）注射两种性别的小鼠（C57 BL/6），并在注射后3、24、48小时和6天制备海马切片。刺激Schaffer侧支，记录锥体细胞层的细胞外诱发电位。的阈值，定义为最小强度的刺激，以唤起0.1 mV的电位，是约5倍以上的metaphit切片比对照切片注射后3小时，然后下降到6天内的控制值。LTP的幅度也放大metaphit在一个时间依赖性的方式。注射后3小时可见效果，在48小时达到最大值，然后在注射后6天下降至略高于对照的水平。这些结果表明，metaphit，一种已知诱导听源性癫痫发作的化合物，能够改变海马中的突触可塑性。本研究的结果也与我们先前的数据一致，这些数据表明LTP和听源性癫痫发作的机制之间存在相互作用。
The influence of metaphit, a phencyclidine derivative, on the amplitude of Long-Term Potentiation (LTP) in the mouse hippocampus was investigated. Mice (C57BL/6) of both sexes were injected with metaphit (80 mg/kg) and hippocampal slices were prepared at 3, 24, 48 hrs and 6 days following injection. The extracellular evoked potentials were recorded from the pyramidal cell layer following Schaffer collateral stimulation. The threshold value, defined as the minimum strength of the stimuli to evoke a 0.1 mV potential, was about 5 fold greater in metaphit slices than in control slices 3 hr following injection, and then declined to the control value within 6 days. The magnitude of LTP was also amplified by metaphit in a time-dependent fashion. The effect was visible three hours after injection, reached its maximum at 48 hr and then declined to a level slightly higher than control at 6 days following injection. These results demonstrate that metaphit, a compound known to induce audiogenic seizures, is able to modify synaptic plasticity in the hippocampus. Presented results are also in agreement with our previous data which demonstrated an interaction between the mechanisms of LTP and audiogenic seizure.