Biochemical selection systems for mammalian cells: The essential amino acids
Biochemical selection systems for mammalian cells: The essential amino acids
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哺乳动物细胞的生化选择系统：必需氨基酸
DOI：
10.1007/bf01542624
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发表时间：
1976
期刊：
影响因子：
--
通讯作者：
R. Klebe
中科院分区：
文献类型：
--
作者：
S. Naylor;Lydia L. Busby;R. Klebe
19种氨基酸衍生物可满足培养的哺乳动物细胞的必需氨基酸需求。这一发现（a）证实了动物营养研究的结果，（B）鉴定了19种必需氨基酸衍生物，并应允许分离一类新的营养缺陷型突变体。本调查中检测到了5种天然存在的营养缺陷型标志物;即，中国仓鼠卵巢（CHO）细胞不能利用胱硫醚和瓜氨酸，HTC+肝癌细胞不能代谢瓜氨酸，并证实了Eagle的观察结果，即KB细胞可以利用高胱氨酸代替甲硫氨酸或胱氨酸和d-胱氨酸代替l-胱氨酸。
The essential amino acid requirement of cultured mammalian cells can be satisfied by 19 amino acid derivatives. This finding (a) confirms the results of animal nutritional studies and (b) identifies 19 essential amino acid derivatives and should permit the isolation of a new class of auxotrophic mutants. Five naturally occurring auxotrophic markers have been detected in this survey; namely, inability to utilize cystathionine and citrulline in Chinese hamster ovary (CHO) cells, inability to metabolize citrulline by HTC+ hepatoma cells, and confirmation of Eagle's observation that KB cells can utilize homocystine in place of methionine or cystine and d-cystine in place of l-cystine.