Drosophila Aversive Behavior toward Erwinia carotovora carotovora Is Mediated by Bitter Neurons and Leukokinin
Drosophila Aversive Behavior toward Erwinia carotovora carotovora Is Mediated by Bitter Neurons and Leukokinin
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DOI：
10.1016/j.isci.2020.101152
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发表时间：
2020-06-26
期刊：
影响因子：
5.8
通讯作者：
Royet, Julien
中科院分区：
文献类型：
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作者：
Charroux, Bernard;Daian, Fabrice;Royet, Julien
植物病原体胡萝卜软腐欧文氏菌（Ecc）已被成功地用于破译调节果蝇和细菌之间相互作用的一些机制，主要是在两个物种之间的强制关联之后。果蝇通常如何感知 Ecc 的存在并做出反应尚不清楚。通过果蝇喂养二选一分析和视频跟踪，我们发现果蝇首先被 Ecc 污染的溶液吸引，但随后又被排斥。最初的吸引阶段依赖于嗅觉 Gr63a 和 Gaq 蛋白，而第二个排斥阶段则需要功能性的味觉系统。基因操作和钙成像表明，厌恶阶段需要苦味神经元和味觉受体 Gr66a 和 Gr33a，并且神经肽白细胞激肽也参与其中。我们还证明这些行为独立于 NF-kappa B 级联，该级联控制着对细菌的一些免疫、代谢和行为反应。
The phytopathogen Erwinia carotovora carotovora (Ecc) has been used successfully to decipher some of the mechanisms that regulate the interactions between Drosophila melanogaster and bacteria, mostly following forced association between the two species. How do Drosophila normally perceive and respond to the presence of Ecc is unknown. Using a fly feeding two-choice assay and video tracking, we show that Drosophila are first attracted but then repulsed by an Ecc-contaminated solution. The initial attractive phase is dependent on the olfactory Gr63a and Gaq proteins, whereas the second repulsive phase requires a functional gustatory system. Genetic manipulations and calcium imaging indicate that bitter neurons and gustatory receptors Gr66a and Gr33a are needed for the aversive phase and that the neuropeptide leukokinin is also involved. We also demonstrate that these behaviors are independent of the NF-kappa B cascade that controls some of the immune, metabolic, and behavioral responses to bacteria.