Electrochemical cholesterol sensor based on cholesterol oxidase and MoS2-AuNPs modified glassy carbon electrode
Electrochemical cholesterol sensor based on cholesterol oxidase and MoS2-AuNPs modified glassy carbon electrode
复制标题
基于胆固醇氧化酶和MoS2-AuNPs修饰玻碳电极的电化学胆固醇传感器
DOI：
10.1016/j.snb.2016.04.019
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发表时间：
2016-10-05
影响因子：
8.4
通讯作者：
Kokot, Serge
中科院分区：
文献类型：
--
作者：
Lin, Xiaoyun;Ni, Yongnian;Kokot, Serge
使用二硫化钼（MoS2）和金纳米粒子（AuNPs）组成的纳米复合混合物成功构建了一种新型修饰电极。研究了其作为胆固醇生物传感器的应用。通过扫描电子显微镜（SEM）、透射电子显微镜（TEM）、原子力显微镜（AFM）、X射线衍射（XRD）和能量色散X射线光谱（EDS）对制备的MoS2-AuNPs纳米颗粒的形貌进行了表征。通过电化学阻抗谱（EIS）和循环伏安法（CV）表征了构建每个阶段的修饰电极的物理化学性质。采用电流法i-t法对胆固醇进行定量分析，胆固醇的校准曲线在0.5-48 M范围内呈线性，检测限(LOD)为0.26 +/- 0.015 μM。表观米氏常数(K-M(app))和灵敏度估计为0.325 mM和4460 μA mM(-1)分别为厘米(-2)。该新型传感器具有令人满意的重现性和稳定性，并成功用于蛋黄和猪肝样品中胆固醇的定量分析。 (C) 2016 Elsevier B.V. 保留所有权利。
A novel modified electrode has been successfully constructed with the use of a nano-composite mixture consisting of molybdenum disulfide (MoS2) and gold nanoparticles (AuNPs). Its application as a cholesterol biosensor was investigated. The morphology of the prepared MoS2-AuNPs nanoparticles were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), atomic force microscope (AFM), X-ray diffraction (XRD) and energy dispersive X-ray spectroscopy (EDS). The physicochemical properties of the modified electrode at each stage of the construction were characterized by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). Quantitative analysis of cholesterol was carried out with the use of the amperometric i-t method, and the calibration plot for cholesterol was linear in the range of 0.5-48 M with a limit of detection (LOD) of 0.26 +/- 0.015 mu M. The apparent Michaelis-Menten constant (K-M(app)) and sensitivity were estimated to be 0.325 mM and 4460 mu A mM(-1) cm(-2), respectively. This novel sensor produced satisfactory reproducibility and stability, and was used successfully for the quantitative analysis of cholesterol in egg yolk and pork liver samples. (C) 2016 Elsevier B.V. All rights reserved.