LACRIMAL PROTEIN SECRETION - COMPARISON OF YOUNG AND OLD RATS
LACRIMAL PROTEIN SECRETION - COMPARISON OF YOUNG AND OLD RATS
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DOI：
10.1016/0014-4835(85)90015-6
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发表时间：
1985-01-01
影响因子：
3.4
通讯作者：
WELCH, MH
中科院分区：
文献类型：
--
作者：
BROMBERG, BB;WELCH, MH
健康4月龄和24月龄泪腺的体外蛋白分泌反应测量老年F344大鼠。将组织碎片在0.2 ml灌注室中孵育，并通过4分钟的0.01 mM卡巴胆碱推注刺激以分泌蛋白质。收集灌流液的级分并测定蛋白质浓度和过氧化物酶活性。卡巴胆碱的反应是一个明确的分泌蛋白质，包括过氧化物酶的峰。该反应被1.0 μ M阿托品抑制。雄性和雌性腺体的反应之间没有差异。幼腺蛋白质分泌高峰含263.9 . ±. 71.8与175.4 μ g/g组织-1相比，56.5对于老年腺体，μ g/g组织-1（P < 0.005）。虽然分泌的过氧化物酶的平均活性是相似的，在2个年龄组，老年腺体显着更多变的反应。F344大鼠泪腺的增龄性变化可能是研究泪腺增龄性变化的一个有用模型。
The in vitro protein secretory response of lacrimal glands from healthy 4- and 24-mo.-old F344 rats was measured. Tissue fragments were incubated in a 0.2 ml perifusion chamber and stimulated to secrete protein by a 4-min bolus of 0.01 mM carbachol. Fractions of the perifusate were collected and assayed for protein concentration and peroxidase activity. The response to carbachol was a well-defined peak of secreted protein that included peroxidase. The response was inhibited by 1.0 .mu.M atropine. No differences were found between the responses of male and female glands. The peak of protein secretion by the young glands contained 263.9 .+-. 71.8 .mu.g g tissue-1 compared to 175.4 .+-. 56.5 .mu.g g tissues-1 for the old glands (P < 0.005). While the mean activity of the secreted peroxidase was similar in the 2 age groups, the aged glands were significantly more variable in their response. The F344 rat may provide a useful model for studying the normal age-dependent changes that occur in lacrimal gland.