Generating mosaics for lineage analysis in flies.
Generating mosaics for lineage analysis in flies.
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DOI：
10.1002/wdev.122
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发表时间：
2014-01
期刊：
影响因子：
--
通讯作者：
Lee T
中科院分区：
文献类型：
--
作者：
Lee T
By generating and studying mosaic organisms we are learning how intricate tissues form as cells proliferate and diversify through organism development. FLP/FRT-mediated site-specific mitotic recombination permits the generation of mosaic flies with efficiency and control. With heat-inducible or tissue-specific FLP transgenes at our disposal, we can engineer mosaics carrying clones of homozygous cells that come from specific pools of heterozygous precursors. This permits detailed cell lineage analysis followed by mosaic analysis of gene functions in the underlying developmental processes. Expression of transgenes (e.g. reporters) only in the homozygous cells enables mosaic analysis in the complex nervous system. Tracing neuronal lineages by using mosaics revolutionized mechanistic studies of neuronal diversification and differentiation, exemplifying the power of genetic mosaics in developmental biology.
