The quaternary structure of Escherichia coli RNA polymerase studied with (scanning) transmission (immuno)electron microscopy.
The quaternary structure of Escherichia coli RNA polymerase studied with (scanning) transmission (immuno)electron microscopy.
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使用（扫描）透射（免疫）电子显微镜研究了大肠杆菌 RNA 聚合酶的四级结构。
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发表时间：
1983
期刊：
影响因子：
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通讯作者：
W. Stender
中科院分区：
文献类型：
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作者：
W. Tichelaar;W. Schutter;A. Arnberg;E. van Bruggen;W. Stender
本文提出了大肠杆菌RNA聚合酶（三磷酸核苷：RNA核苷酸转移酶，EC 2.7.7.6）四级结构的模型。它是基于酶分子谱分类和免疫电子显微镜应用的结果。分子的分类，准备与单碳层技术，首先实现了在暗场记录的图像与扫描透射电子显微镜，后来在明场透射电子显微镜记录的图像。它导致五个大致相等大小的组，包含约80%的核心酶谱。全酶谱可以分为相同的类别，并且具有近似相同的尺寸（9 nm X 16 nm）。根据分类图谱的形状和大小，初步构建了核心酶的模型。该模型的阴影投影与针对α、β、β '和σ的抗体的附着位点在分布图上的分布的相关性已经导致了其中亚基被定位的核心和全酶的模型。该模型与文献数据的四级结构的RNA聚合酶。
A model for the quaternary structure of Escherichia coli RNA polymerase (nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) is presented. It is based on results from classification of profiles of enzyme molecules, and from application of immuno electron microscopy. Classification of molecules, prepared with the single carbon layer technique, was first achieved for images recorded in dark field with the scanning transmission electron microscope and later on for images recorded in bright-field transmission electron microscopy. It results in five approximately equally sized groups, containing about 80% of the core enzyme profiles. Holoenzyme profiles can be grouped into the same classes, and have approximately the same dimensions (9 nm X 16 nm). Based on the shapes and sizes of the classified profiles, a tentative model for core enzyme has been constructed. Correlation of shadow projections of this model, with the distributions of attachment sites of antibodies against alpha, beta, beta' and sigma over the profiles, has led to models for core and holoenzyme in which the subunits are localized. The model is compared with literature data on the quaternary structure of RNA polymerase.