Optimization of Liquid Fermentation Medium for Production of Inonotus sanghuang (Higher Basidiomycetes) Mycelia and Evaluation of their Mycochemical Contents and Antioxidant Activities.
Optimization of Liquid Fermentation Medium for Production of Inonotus sanghuang (Higher Basidiomycetes) Mycelia and Evaluation of their Mycochemical Contents and Antioxidant Activities.
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DOI：
10.1615/intjmedmushrooms.v17.i7.80
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发表时间：
2015
影响因子：
1.2
通讯作者：
Xue-Mei Tian;Yu Dai;Ai-Rong Song;Kun Xu;L. Ng
中科院分区：
文献类型：
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作者：
Xue-Mei Tian;Yu Dai;Ai-Rong Song;Kun Xu;L. Ng
桑黄桦褐孔菌（Inonotus sanghuang）是一种具有重要药理活性的传统中药“桑黄”菌。本研究旨在优化I.研究了摇瓶培养和静置培养对桑黄菌丝体总黄酮、总酚、多糖产量及抗氧化活性的影响。桑黄菌丝提取物（ISME）。在优化的培养基组成（玉米粉38.96 g/L，酵母膏4.15 g/L，麸皮20.55 g/L，pH 6.39）下，预测和实验最佳菌丝体生物量分别为17.60 g/L和18.33±0.86 g/L。两段培养结果表明，菌丝体中总黄酮和总酚含量分别提高了37.92%和77.27%。然而，不规则的多糖含量，注意到整个实验期间。抗氧化实验表明，ISME具有良好的清除自由基的活性，这主要是由多酚类代谢产物。
Inonotus sanghuang, an authentic "Sanghuang" mushroom used in traditional Chinese medicine, is known to possess important pharmacological activities. In this study, we aimed to optimize the liquid fermentation medium for I. sanghuang mycelial production and to determine the effects of two-stage cultivation (shake and static) on the yield of total flavonoids, total phenolics, and polysaccharides, as well as the antioxidant activities of I. sanghuang mycelial extracts (ISME). Under an optimized medium composition (38.96 g/L of corn flour, 4.15 g/L of yeast extract, 20.55 g/L of bran and pH 6.39), the predicted and experimental optimal mycelial biomasses were 17.60 g/L and 18.33±0.86 g/L, respectively. The results of two-stage cultivation showed that contents of total flavonoids and total phenolics in mycelia increased by 37.92% and 77.27%, respectively. However, irregular polysaccharide contents were noted throughout the experimental period. Antioxidant assays showed that ISME possessed good free-radical scavenging activity, which is mainly contributed by polyphenolic-type metabolites.