Genome-Wide Analysis of the Indispensable Role of Non-structural Proteins in the Replication of SARS-CoV-2.
Genome-Wide Analysis of the Indispensable Role of Non-structural Proteins in the Replication of SARS-CoV-2.
复制标题
非结构蛋白在 SARS-CoV-2 复制中不可或缺作用的全基因组分析
DOI：
10.3389/fmicb.2022.907422
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发表时间：
2022
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
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作者：
了解SARS-CoV-2的复制和转录过程对制定抗病毒策略至关重要。复制酶多蛋白对病毒复制是必不可少的。然而，是否SARS-CoV-2复制酶多蛋白衍生的所有nsps都是必不可少的尚不完全清楚。在本研究中，我们以SARS-CoV-2复制子为系统，研究了每个nsp在病毒复制中的作用。我们发现，除nsp16外，所有的nsp缺失都严重损害了复制子的复制，而nsp14可以恢复其在病毒复制子中缺失所导致的复制缺陷。由于nsp1、nsp3和nsp16的表达失败，我们无法得出它们的反式救援功能的结论。我们的研究为了解nsp在病毒复制和转录中的作用提供了一个新的角度，有助于评估nsp作为抗病毒药物开发的靶点。
Understanding the process of replication and transcription of SARS-CoV-2 is essential for antiviral strategy development. The replicase polyprotein is indispensable for viral replication. However, whether all nsps derived from the replicase polyprotein of SARS-CoV-2 are indispensable is not fully understood. In this study, we utilized the SARS-CoV-2 replicon as the system to investigate the role of each nsp in viral replication. We found that except for nsp16, all the nsp deletions drastically impair the replication of the replicon, and nsp14 could recover the replication deficiency caused by its deletion in the viral replicon. Due to the unsuccessful expressions of nsp1, nsp3, and nsp16, we could not draw a conclusion about their in trans-rescue functions. Our study provided a new angle to understand the role of each nsp in viral replication and transcription, helping the evaluation of nsps as the target for antiviral drug development.