A rat head holder for simultaneous scanning of two rats in small animal PET scanners: Design, construction, feasibility testing and kinetic validation
A rat head holder for simultaneous scanning of two rats in small animal PET scanners: Design, construction, feasibility testing and kinetic validation
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DOI：
10.1016/j.jneumeth.2008.08.031
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发表时间：
2009-01-15
影响因子：
3
通讯作者：
Morris, Evan D.
中科院分区：
文献类型：
--
作者：
Cheng, Tee Ean;Yoder, Karmen K.;Morris, Evan D.
为了降低成像成本，我们设计了一个头部保持器，用于在小动物PET扫描仪中同时扫描两只大鼠。我们的目标是（i）保持高灵敏度和（ii），以尽量减少重新定位之间的scannes.Methods错误：半立体定位双大鼠头部保持器的设计和构造双大鼠扫描在我们的IndyPET-II扫描仪和商业microPET P4。它还用于在小口径、高分辨率动物扫描仪（“ISAP”）中对单个大鼠进行扫描。通过在不同日期对单只大鼠进行多次[C-11]雷氯必利扫描，验证位置重复性。重新定位的准确性通过视觉和通过图像对准获得的度量来确定。为了比较图像，通过分析六只大鼠的[F-18]氟脱氧葡萄糖（[F-18]FDG）动态PET研究来评估动力学验证。每只大鼠扫描两次：一次是单独的，大脑位于视野中心（CFOV），一次是与伴侣一起，大脑远离CFOV。在每次双鼠训练期间，两只大鼠都注射FDG。从全脑图像计算Patlak摄取常数（Ki）。衰减和散射校正对单与双扫描images.Results的影响进行了探讨：图像比较和对齐指标表明大鼠良好的重新定位。单次和双次大鼠扫描的标度时间-活性曲线无法区分。结论：在半立体定位保持器中进行大鼠双扫描是一种经济实用的小动物扫描方法，且不影响[F-18]FDG动态扫描数据的动力学准确性。(C)2008 Elsevier B. V.保留所有权利。
To reduce imaging costs, we designed a head holder for scanning two rats simultaneously in small animal PET scanners. Our goals were (i) to maintain high sensitivity and (ii) to minimize repositioning error between scans.Methods: A semi-stereotaxic dual rat head holder was designed and constructed for dual rat scanning in our IndyPET-II scanner and the commercial microPET P4. It was also used for single rat scanning in a small-bore, high-resolution animal scanner ("ISAP"). Positional repeatability was validated via multiple [C-11]Raclopride scans of a single rat on different days. Accuracy of repositioning was determined by visual and by metrics derived through image alignment. comparison of images,Kinetic validation was assessed via analysis of [F-18]Fluorodeoxyglucose ([F-18]FDG) dynamic PET studies of six rats. Each rat was scanned twice: once individually, with brain positioned at the center of field of view (CFOV), and once with a partner, with brain away from CFOV. Both rats were injected with FDG during each dual rat session. Patlak uptake constants (Ki) were calculated from whole brain images. Effects of attenuation and scatter correction on single versus dual scan images were explored.Results: Image comparison and alignment metrics indicated excellent repositioning of rats. Scaled time-activity-curves from single and dual rat scans were indistinguishable. Average single and dual scan Ki values differed by only 6.3 +/- 7.5%.Conclusion: Dual rat scanning in a semi-stereotaxic holder is practical for economical small animal scanning and does not compromise kinetic accuracy of [F-18]FDG dynamic scan data. (C) 2008 Elsevier B.V. All rights reserved.