Bi-directional transport of GLUT4 vesicles near the plasma membrane of primary rat adipocytes.
Bi-directional transport of GLUT4 vesicles near the plasma membrane of primary rat adipocytes.
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DOI：
10.1016/j.bbrc.2007.05.075
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发表时间：
2007-07
影响因子：
3.1
通讯作者：
Yingke Xu;Ke-di Xu;Jie-yue Li;Linqing Feng;D. Lang;Xiao-Xiang Zheng
中科院分区：
文献类型：
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作者：
Yingke Xu;Ke-di Xu;Jie-yue Li;Linqing Feng;D. Lang;Xiao-Xiang Zheng
胰岛素通过将含有GLUT 4蛋白的细胞内膜囊泡动员到质膜上来刺激脂肪细胞对葡萄糖的摄取。在这里，我们采用时间推移全内反射荧光显微镜研究运动参数和特性的外源性表达GLUT 4囊泡在基础，胰岛素和诺考达唑处理的原代大鼠脂肪细胞。我们的研究结果表明，微管是必不可少的长距离运输的GLUT 4囊泡，但不是强制性的GLUT 4分布在大鼠脂肪细胞。胰岛素降低了囊泡的流动性，使它们束缚/停靠在PM上，并最终具有组成性胞吐作用。此外，长距离双向运动的GLUT 4囊泡是可视化的第一次由TIRFM。提示胰岛素信号通路与微管之间可能存在相互作用，从而调控脂肪细胞GLUT 4的转位。
Insulin stimulates glucose uptake into adipocytes by mobilizing intracellular membrane vesicles containing GLUT4 proteins to the plasma membrane. Here we applied time-lapse total internal reflection fluorescence microscopy to study moving parameters and characters of exogenously expressed GLUT4 vesicles in basal, insulin and nocodazole treated primary rat adipocytes. Our results showed that microtubules were essential for long-range transport of GLUT4 vesicles but not obligatory for GLUT4 distribution in rat adipocytes. Insulin reduced the mobility of the vesicles, made them tethered/docked to the PM and finally had constitutive exocytosis. Moreover, long-range bi-directional movements of GLUT4 vesicles were visualized for the first time by TIRFM. It is likely that there are interactions between insulin signaling and microtubules, to regulating GLUT4 translocation in rat adipocytes.