Interaction of cyclic AMP receptor protein with the ilvB biosynthetic operon in E. coli.
Interaction of cyclic AMP receptor protein with the ilvB biosynthetic operon in E. coli.
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环 AMP 受体蛋白与大肠杆菌中 ilvB 生物合成操纵子的相互作用。
DOI：
10.1093/nar/12.21.8145
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发表时间：
1984
影响因子：
14.9
通讯作者：
Freundlich,M
中科院分区：
文献类型：
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作者：
Friden,P;Tsui,P;Okamoto,K;Freundlich,M
dna酶和限制性内切位点保护研究表明，cAMP及其受体蛋白（CRP）在大约−44至−82位置与它们的il - vboperon启动子结合。该区域包含与其他crp依赖启动子同源的序列。通过添加cAMP和CRP， il - vbpromoter的体外转录显著增加。当ilvB模板缺乏所提出的CRP结合位点时，没有发现这种刺激。cAMP-CRP并没有改变其ilvbleader内的转录终止程度，这表明该调控系统可能独立于该操纵子负调控所涉及的衰减机制。限制性内切酶位点保护研究和改变启动子片段的实验结果表明，CRP刺激其ilvboperon的机制可能与最近提出的forlac模型相似(1)。
DNase and restriction site protection studies show that cAMP and its receptor protein (CRP) bind to the promoter of theilvBoperon at approximately position −44 to −82. This region contains sequences that are homologous to those found in other CRP-dependent promoters.Invitrotranscription from theilvBpromoter was markedly increased by the addition of cAMP and CRP. This stimulation was not found when the ilvB template lacked the proposed CRP binding site. cAMP-CRP did not alter the extent of transcription termination within theilvBleader suggesting that this regulatory system may be independent of the attenuation mechanism involved in the negative control of this operon. The results of restriction enzyme site protection studies and experiments with altered promoter fragments indicate that the mechanism for CRP stimulation of theilvBoperon may be similar to a model recently proposed forlac(1).
“1972政权”日本安保体系下的台湾（中文）
DOI：
--
发表时间：
2022
期刊：
影响因子：
--
作者：
Barrett;B;Ohta;H.;and Trencher;G. "Prospects for Acceleration of Socio-Technical Transitions for Deep Decarbonization," pp. 400-414;青山瑠妙;福田円;舒旻「中国：EUとの脆弱な相互依存」pp. 247-271;福田円;青山瑠妙;青山瑠妙;青山瑠妙;福田円;青山瑠妙;福田円
通讯作者：
福田円