Biological control of strawberry gray mold by Clonostachys rosea under field conditions
Biological control of strawberry gray mold by Clonostachys rosea under field conditions
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DOI：
10.1016/j.biocontrol.2008.04.023
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发表时间：
2008-09-01
影响因子：
4.2
通讯作者：
Antunes, Ricardo F.
中科院分区：
文献类型：
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作者：
Cota, Luciano V.;Maffia, Luiz A.;Antunes, Ricardo F.
灰霉病是由灰葡萄孢 (Botrytis cinerea) 引起的，是巴西重要的草莓病害。作为疾病管理计划的一部分，我们一直在评估玫瑰色克隆竹 (Clonostachysrosea) 的病原体生物控制，并选择了四种分离株作为灰霉病菌的潜在拮抗剂。 2006 年和 2007 年，在田间条件下，我们比较了四种 C Rosea 分离株（每周施用一次或两次）与每周喷洒腐霉利与船长交替喷洒控制灰霉病的效果。施用后至收获前，我们每周进行评估：叶面积 C. Rosea (LAC) 定殖、叶上 B. cinerea 分生孢子梗的平均数量 (ANC)、花 (Iflower) 和果实 (Ifruit) 灰霉病发生率、果实潜伏感染发生率 (Ilat) 和产量。每周两次施用玫瑰花提供了较高的 LAC（16.97%）、较小的 ANC（10.28；在检查处理中为 78.22，喷水）、较小的 IFlower（10.02%；在检查处理中为 50.55%）和较小的 lFruit（5.95%；在检查处理中为 25.10%）。每周两次施用 C.rosea 时，产量范围在 3490 至 3750 g 之间（-1），而在检查处理中，产量范围在 1740 至 1910 克（-1）之间。 Ilat 在检查处理中为 20%，在其他处理中低于 10%。根据这项为期 2 年的研究，成功的灰霉病管理计划需要每周至少施用 C.rosea 两次。 (C) 2008 Elsevier Inc. 保留所有权利。
Gray mold, caused by Botrytis cinerea, is an important strawberry disease in Brazil. As a component of a disease management program, we have been evaluating pathogen biological control with Clonostachys rosea, and selected four isolates as potential antagonists to B. cinerea. In 2006 and 2007, under field conditions, we compared the efficiency of the four C rosea isolates (applied once or twice a week) with a weekly spray of procymidone alternated with captain in controlling gray mold. Following the applications and up to harvest, we evaluated weekly: leaf area colonization by C. rosea (LAC), average number of B. cinerea conidiophores on leaves (ANC), incidence of gray mold on both flowers (Iflower) and fruits (Ifruit), incidence of latent infections on fruits (Ilat), and yield. The applications of C. rosea twice a week provided higher LAC (16.97%), smaller ANC (10.28; 78.22 in the check treatment, sprayed with water), smaller IFlower (10.02%; 50.55% in the check treatment), and smaller lFruit (5.95%; 25.10% in the check treatment). Yield ranged between 3490 and 3750 g plot(-1) with applications of C. rosea twice a week and between 1740 and 1910g plot(-1) in the check treatment. Ilat was 20% in the check treatment and less than 10% in the other treatments. Based on this 2-year study, at least two weekly applications of C. rosea are required for a successful gray mold management program. (C) 2008 Elsevier Inc. All rights reserved.