A 2-D gel reference map of the basic human heart proteome
A 2-D gel reference map of the basic human heart proteome
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DOI：
10.1002/pmic.201000182
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发表时间：
2011-09-01
期刊：
影响因子：
3.4
通讯作者：
Dunn, Michael J.
中科院分区：
文献类型：
--
作者：
Polden, Julie;McManus, Ciara A.;Dunn, Michael J.
我们用2-DE对人心脏碱性蛋白(pH 6-11)进行了鉴定。采用纸桥装载法分离人左心左室心肌组织，在pH 6~11的IPG纸条上第一维分离蛋白质，然后用12%的十二烷基硫酸钠-聚丙烯酰胺凝胶进行第二维分离。然后用质谱仪鉴定蛋白质，并使用内部开发的蛋白质组数据分析平台Proline进行分析。该蛋白质组图谱包含有151种独特蛋白质的176个识别点，已作为UCD-2DPAGE数据库的一部分在http://proteomics-portal.ucd.ie:8082.上提供相关的质谱学数据已提交给Pride(登录号#10098)。这一参考图以及UCD-2DPAGE数据库提供的其他心脏参考图，将有助于进一步研究扩张型心肌病和缺血性心脏病等心脏疾病的蛋白质组学。
We have undertaken the identification of basic proteins (pH 6-11) of the human heart using 2-DE. Tissue from the left ventricle of human heart was lysed and proteins were separated in the first dimension on pH 6-11 IPG strips using paper-bridge loading followed by separation on 12% SDS polyacrylamide gels in the second dimension. Proteins were then identified by mass spectrometry and analysed using Proline, a proteomic data analysis platform that was developed in-house. The proteome map contains 176 identified spots with 151 unique proteins and has been made available as part of the UCD-2DPAGE database at http://proteomics-portal.ucd.ie:8082. The associated mass spectrometry data have been submitted to PRIDE (Accession number #10098). This reference map, and the other heart reference maps available through the UCD-2DPAGE database, will aid further proteomic studies of heart diseases such as dilated cardiomyopathy and ischaemic heart disease.