Tracking the green invaders: advances in imaging virus infection in plants.

Tracking the green invaders: advances in imaging virus infection in plants.
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追踪绿色入侵者:植物病毒感染成像的进展。

DOI:
10.1042/bj20100372
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发表时间:
2010
期刊:
The Biochemical journal
影响因子:
--
通讯作者:
Tilsner J
Tilsner J
中科院分区:
--
文献类型:
--
作者:
Tilsner J

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生物成像对我们了解植物病毒感染有重要贡献。在目前的审查,我们描述的技术进步,使成像的感染过程在以前无法达到的水平。我们强调这些亚细胞成像的新进展如何有助于对病毒感染过程的所有阶段进行详细解剖。具体来说,我们专注于:(i)通过多样化的细胞标记实现病毒蛋白的越来越详细的定位;(ii)利用荧光显微镜对蛋白质进行体内功能分析的方法;(iii)病毒rna成像;(四)在光学显微镜和电子显微镜之间架起桥梁的方法;(5)模糊了成像生物学和结构生物学之间区别的方法。我们描述了这些技术的优点和缺点,并将它们放在分析植物病毒感染的更广泛的角度来看。
Bioimaging contributes significantly to our understanding of plant virus infections. In the present review, we describe technical advances that enable imaging of the infection process at previously unobtainable levels. We highlight how such new advances in subcellular imaging are contributing to a detailed dissection of all stages of the viral infection process. Specifically, we focus on: (i) the increasingly detailed localizations of viral proteins enabled by a diversifying palette of cellular markers; (ii) approaches using fluorescence microscopy for the functional analysis of proteinsin vivo; (iii) the imaging of viral RNAs; (iv) methods that bridge the gap between optical and electron microscopy; and (v) methods that are blurring the distinction between imaging and structural biology. We describe the advantages and disadvantages of such techniques and place them in the broader perspective of their utility in analysing plant virus infection.
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