Ablation of eosinophil and IgE responses with anti-IL-5 or anti-IL-4 antibodies fails to affect immunity against Schistosoma mansoni in the mouse.
Ablation of eosinophil and IgE responses with anti-IL-5 or anti-IL-4 antibodies fails to affect immunity against Schistosoma mansoni in the mouse.
复制标题
用抗 IL-5 或抗 IL-4 抗体消除嗜酸性粒细胞和 IgE 反应并不能影响小鼠对曼氏血吸虫的免疫力。
DOI：
10.4049/jimmunol.145.11.3911
复制
发表时间：
1990
影响因子：
4.4
通讯作者：
A. Cheever
中科院分区：
文献类型：
--
作者：
A. Sher;R. Coffman;S. Hieny;A. Cheever
为探讨过敏性免疫反应在血吸虫病保护性免疫中的作用，对接种曼氏血吸虫尾蚴的小鼠在攻击感染前和攻击期间分别用抗IL-5或IL-4的中和性单抗进行治疗。抗IL-5处理的免疫小鼠表现出对皮肤和肺部迁移的血吸虫炎症反应中存在的循环和组织中的嗜酸性粒细胞的完全消融，但仍然像用对照单抗处理的接种动物一样有效地消除了挑战感染。类似地，用抗IL-4单抗治疗接种了疫苗的小鼠显著降低了血清IgE，但未能降低免疫力。在第二种模型中，也评估了抗IL-5介导的嗜酸性粒细胞耗竭的效果，在该模型中，伴随的慢性感染诱导了耐药性。同样，在没有循环和组织嗜酸性粒细胞的情况下，观察到正常的、未改变的保护作用。与上述发现相反，抗干扰素-γ治疗被发现导致接种疫苗的小鼠部分免疫衰竭，而与之相反的是，增加了对肺部血吸虫入侵的炎症反应的数量。这些观察结果反对在接种辐射尾蚴疫苗诱导的抗血吸虫免疫中需要嗜酸性粒细胞或IgE，或者在先前感染的小鼠的抵抗中需要嗜酸性粒细胞，并支持先前的数据，即干扰素-γ依赖的细胞介导的效应机制在疫苗诱导的抵抗中起作用。
To investigate the role of anaphylactic immune responses in protective immunity against schistosomiasis, mice vaccinated with irradiated cercariae of Schistosoma mansoni were treated with neutralizing mAb antibodies against either IL-5 or IL-4 before and during challenge infection. Anti-IL-5-treated vaccinated mice showed a complete ablation of circulating as well as tissue eosinophils present in inflammatory reactions to migrating schistosomula in the skin and lungs but nevertheless eliminated challenge infections as effectively as vaccinated animals treated with a control mAb. Similarly, treatment of vaccinated mice with an anti-IL-4 mAb markedly reduced serum IgE although failing to diminish immunity. The effect of anti-IL-5 mediated eosinophil depletion was also assessed in a second model in which resistance is induced by concomitant chronic infection. Again, normal, unaltered protection was observed in the absence of circulating and tissue eosinophils. In contrast to the above findings, treatment with anti-IFN-gamma was found to cause a partial depletion of immunity in vaccinated mice whereas, paradoxically, increasing the numbers of inflammatory reactions against invading schistosomula in the lungs. These observations argue against a requirement for either eosinophils or IgE in the anti-schistosome immunity induced by vaccination with irradiated cercariae or for eosinophils in the resistance resulting from previous infection in mice and support previous data suggesting a role for an IFN-gamma dependent cell-mediated effector mechanism in vaccine-induced resistance.