Introduction of Functional RNA into Plant Protoplasts by Electroporation
Introduction of Functional RNA into Plant Protoplasts by Electroporation
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通过电穿孔将功能性 RNA 引入植物原生质体
DOI：
10.1093/oxfordjournals.pcp.a077141
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
I. Takebe
中科院分区：
文献类型：
--
作者：
K. Okada;T. Nagata;I. Takebe
本文介绍了一种在分光光度计比色皿内的两电极间产生不同强度放电的装置。利用该装置研究了利用烟草花叶病毒（TMV）和黄瓜花叶病毒（CMV）RNA将功能性RNA有效导入植物原生质体的条件。在最佳条件下的电穿孔导致80%的烟草细胞系BY2的原生质体中产生TMV和CMV。烟草悬浮培养原生质体的TMVRNA感染率为60%，烟草叶肉原生质体的TMVRNA感染率为40%。在TMV和CMV颗粒存在下的电穿孔也导致感染，表明形成大于30 nm的孔。介绍了电导入法的优点和用途。
A simple apparatus was constructed for producing electric discharge of varying intensities between two electrodes in a spectrophotometer cuvette. This apparatus was used to study the conditions for efficient introduction of functional RNA into plant protoplasts using RNAs of tobacco mosaic virus (TMV) and cucumber mosaic virus (CMV). Electroporation under optimal conditions resulted in the production of TMV and CMV in 80% of the protoplasts from tobacco cell line BY2. Infection by TMVRNA occurred in 60% ofVinca roseasuspension culture protoplasts and in 40% of tobacco mesophyll protoplasts. Electroporation in the presence of TMV and CMV particles also resulted in infection, suggesting that pores larger than 30 nm are formed. The advantages and uses of the electrical method for introduction of functional RNA are discussed.