BMP-2 incorporated in a tricalcium phosphate bone substitute enhances bone remodeling in sheep
BMP-2 incorporated in a tricalcium phosphate bone substitute enhances bone remodeling in sheep
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DOI：
10.1177/0885328207083311
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发表时间：
2008-05-01
影响因子：
2.9
通讯作者：
Niedhart, Christopher
中科院分区：
文献类型：
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作者：
Maus, Uwe;Andereya, Stefan;Niedhart, Christopher
骨形态发生蛋白-2（BMP-2）是一种已知的骨诱导蛋白，其需要载体才能局部应用。作为前述载体的替代品，测试了原位硬化、可吸收和骨传导性β-磷酸三钙骨水泥（TCP）。牛股骨远端骨骺的钻孔缺损用复合材料填充，复合材料由TCP和200 μ g rhBMP-2/cm（3）TCP、自体骨移植物、纯TCP组成，或留空。在7周和3个月后进行放射学随访。对绵羊实施安乐死，并通过显微放射照相术、组织学和组织形态计量学分析骨样品。显微放射照相术和组织学显示纯TCP和复合材料的类似结果。缺损处填充有骨小梁，新形成的骨与剩余的TCP颗粒紧密接触。大部分骨水泥被再吸收，在复合材料组中，剩余骨水泥颗粒的量减少。用自体骨移植物治疗的缺损被完全填充，而未治疗的缺损仅显示少量源自缺损边缘的骨。与复合材料或自体骨移植物治疗的缺损相比，纯TCP治疗的缺损的组织形态学显示骨含量显著增加。剩余骨水泥颗粒的分析显示，与纯TCP相比，TCP/rhBMP-2组中的骨水泥显著较少。rhBMP-2组中的骨和骨水泥含量总和显示与自体骨移植后发现的钙化结构相当。与自体骨移植物相比，将rhBMP-2添加到TCP中导致更快的缺损重建，而用纯TCP治疗的缺损不能完全重建。
Bone morphogenetic protein-2 (BMP-2) is a well-known osteoinductive protein, which requires a carrier for local application. As an alternative to the previously described carriers, an in situ hardening, resorbable, and osteoconductive beta-tricalcium phosphate cement (TCP) is tested. Trepanation defects in the bovine distal femoral epiphysis are filled with a composite consisting of TCP and 200 mu g rhBMP-2 per cm(3) TCP, autologous bone graft, pure TCP, or left empty. A radiological follow-up is performed after 7 weeks and 3 months. The sheep are euthanized and bone samples are analyzed by microradiography, histology, and histomorphometry. Microradiography and histology show similar results for pure TCP and the composite. The defects are filled with trabecular bone and newly formed bone is in close contact with the remaining TCP-particles. The majority of the cement is resorbed, in the composite group the amount of remaining cement particles is reduced. Defects treated with autologous bone graft are filled completely, while untreated defects shows only a small amount of bone originating from the rim of the defect. Histomorphometry of the defects treated with pure TCP shows a significantly increased bone content in comparison to defects treated with the composite or autologous bone graft. Analysis of the remaining cement particles shows significantly less cement in the TCP/rhBMP-2 group in comparison to pure TCP. The sum of bone and cement content in the rhBMP-2 group shows amounts comparable to the calcified structures found following autologous bone grafting. The addition of rhBMP-2 to the TCP leads to faster remodeling of the defect comparable to autologous bone graft, while defects treated with pure TCP are not completely remodeled.