Highly Variable Patterns of Antimicrobial Resistance in Commensal Escherichia coli Isolates from Pigs, Sympatric Rodents, and Flies
Highly Variable Patterns of Antimicrobial Resistance in Commensal Escherichia coli Isolates from Pigs, Sympatric Rodents, and Flies
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DOI：
10.1089/mdr.2009.0913
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发表时间：
2009-09-01
影响因子：
2.6
通讯作者：
Klimes, Jiri
中科院分区：
文献类型：
--
作者：
Literak, Ivan;Dolejska, Monika;Klimes, Jiri
基于抗菌素耐药性基因、整合子和宏观限制性 DNA 图谱，对来自捷克共和国两个具有不同抗生素暴露史的大型农场的猪、同域啮齿动物和苍蝇的抗菌素耐药大肠杆菌菌株进行了表征。根据标准纸片扩散法测试大肠杆菌分离株对 12 种抗菌剂的敏感性。在耐药菌株中，聚合酶链反应用于检测抗生素耐药基因、整合酶基因和基因盒。脉冲场凝胶电泳 (PFGE) 用于大肠杆菌的分子亚型分析。在农场 A（仅长期使用阿莫西林）中，从仔猪、母猪、同域啮齿动物和苍蝇中分离出来的大肠杆菌分别有 75% (n = 198)、65% (n = 49)、11% (n = 139) 和 82% (n = 177) 对抗生素具有耐药性。在农场 B（常用的各种抗生素）中，从仔猪、母猪和同域啮齿动物中分离出来的大肠杆菌分别有 53% (n = 154)、69% (n = 98) 和 54% (n = 74) 对抗生素产生耐药性。在两个农场中，对四环素的耐药率最高，并且菌株的耐药模式差异很大。在猪和果蝇中发现了具有相同抗性表型、基因和 PFGE 谱的分离株。啮齿类动物的分离物显示出独特的 PFGE 特征。同域啮齿动物和苍蝇与猪或其产品的密切接触与啮齿动物和苍蝇带有耐药细菌的定植或猪肠道菌群中发现的耐药基因的转移有关。
Antimicrobial-resistant Escherichia coli strains from pigs, sympatric rodents, and flies from two large farms in the Czech Republic with different antibiotic exposure histories were characterized based on antimicrobial resistance genes, integrons, and macrorestriction DNA profiles. Isolates of E. coli were tested for susceptibility to 12 antimicrobial agents according to the standard disk diffusion method. In resistant isolates, polymerase chain reaction was used to detect antibiotic resistance genes, integrase genes, and gene cassettes. Pulsed-field gel electrophoresis (PFGE) was used for molecular subtyping of E. coli. In farm A (long-term use of amoxicillin only), 75% (n = 198), 65% (n = 49), 11% (n = 139), and 82% (n = 177) of E. coli isolates from piglets, sows, sympatric rodents, and flies, respectively, were antibiotic resistant. In farm B (various antibiotics commonly used), 53% (n = 154), 69% (n = 98), and 54% (n = 74) of E. coli isolates from piglets, sows, and sympatric rodents, respectively, were antibiotic resistant. In both farms, the highest resistance prevalence was to tetracycline, and resistance patterns of isolates were greatly variable. Isolates with the same resistance phenotype, genes, and PFGE profile were found in pigs and flies. Isolates from rodents showed unique PFGE profiles. Close contact of sympatric rodents and flies with pigs or their products was associated with colonization of rodents and flies with resistant bacteria or transfer of resistance genes found in pig intestinal flora.