3D direct writing egg white hydrogel promotes diabetic chronic wound healing via self-relied bioactive property
3D direct writing egg white hydrogel promotes diabetic chronic wound healing via self-relied bioactive property
复制标题
3D直写蛋清水凝胶通过自力生物活性促进糖尿病慢性伤口愈合
DOI：
10.1016/j.biomaterials.2022.121406
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发表时间：
2022-02-16
期刊：
影响因子：
14
通讯作者：
Chang, Qiang
中科院分区：
文献类型：
--
作者：
Guo, Lingling;Niu, Xingtang;Chang, Qiang
近年来的研究表明，水凝胶联合药物或生长因子治疗糖尿病慢性创面取得了显著的效果。然而，这些技术仍然不能令人满意，而且受到高成本、复杂的提纯或制造、复杂的药物加载以及缺乏多功能治疗效果的阻碍。通过对新鲜蛋清的可行加工，提出了一种天然水凝胶，利用其丰富的蛋白质含量和物理交联性，展示了不同种类的蛋清的凝胶过程，在阳离子溶液渗透后，可以将蛋清水凝胶捏合到所需的机械强度，并通过3D打印机直接写入定义良好的结构。同时，该水凝胶具有固有的生物活性成分，可以刺激成纤维细胞和脂肪组织来源的干细胞在增殖、迁移和无细胞毒性方面发挥作用，具有与Matrigel相似的细胞友好底物。此外，3D打印水凝胶实现了促血管生成效应，并促进了体内胶原的沉积，促进了正常和糖尿病慢性创面在没有外源生长因子的情况下的修复。总而言之，这种源自丰富天然食物的水凝胶平台为慢性伤口愈合提供了一种经济而高效的解决方案，并可能在其他生物用途和临床实践中找到更多的治疗作用。
Recent developments have evidenced the remarkable achievements of hydrogel combining drugs or growth factors delivery on diabetic chronic wound management. However, these techniques remain unsatisfactory and are impeded by high-cost, complex purification or fabrication, sophisticated drug loading, and lacking versatile therapeutic effects into one platform. Herein, a natural hydrogel is presented through feasible processing of fresh egg white, benefiting from the abundant protein contents and physical crosslinking, the gelation procedure of egg white from diverse species was demonstrated by the presented method, the egg white hydrogel can be kneaded to desired mechanical strength after infiltration of cation solution and directly wrote to well-defined architecture by the 3D printer. Meanwhile, the hydrogel possessed the inherent bioactive elements that stimulated the fibroblasts and adipose tissue-derived stem cells at the aspect of proliferation, migration, and functions without cytotoxicity which featured a similar cell-friendly substrate as Matrigel. Otherwise, the resulting 3D-printed hydrogels realized a proangiogenic effect and enhanced collagen deposition in vivo to promote the recovery of the normal and diabetic chronic wound in the absence of exogenous growth factors. Collectively, this hydrogel platform derived from abundant natural food provides an economical yet highly effective solution for chronic wound healing and may find more therapeutic roles in other biological utilizations and clinical practices.