EXPRESSION OF NEURONAL NICOTINIC ACETYLCHOLINE-RECEPTOR SUBUNIT GENES IN THE RAT AUTONOMIC NERVOUS-SYSTEM
EXPRESSION OF NEURONAL NICOTINIC ACETYLCHOLINE-RECEPTOR SUBUNIT GENES IN THE RAT AUTONOMIC NERVOUS-SYSTEM
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DOI：
10.1111/j.1460-9568.1994.tb00290.x
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发表时间：
1994-03-01
影响因子：
3.4
通讯作者：
CACHELIN, AB
中科院分区：
文献类型：
--
作者：
RUST, G;BURGUNDER, JM;CACHELIN, AB
在自主神经系统中，传出信号在交感神经节和副交感神经节传递。突触前和突触后神经元之间的快速突触传递是由神经元烟碱乙酰胆碱受体（nAChRs）实现的。对于这些受体的亚基组成仍然知之甚少。建立天然神经元nachr的亚基组成对于了解其在体内和在异源表达系统中表达后的功能特性具有重要意义。我们结合原位杂交和放射自显影来检测在交感和副交感神经节中编码神经元nachr亚基的mrna的存在。射线自显像检查显示，5种核探针（alpha3、alpha4 - 1、alpha7、beta2和beta4）的杂交信号明显高于用义核探针获得的非特异性信号。alpha7的分布具有组织依赖性：在颈上神经节、肾上腺髓质和睫状神经节的神经元中检测到alpha7核蛋白探针结合。相比之下，alpha7杂交信号仅在蝶腭神经节和耳神经节的一小部分神经元中发现（1 - 3%）。我们的结果与alpha3 mRNA表达水平略高于alpha7、alpha4 - 1、beta2和beta4的观点一致。
In the autonomic nervous system efferent signals are relayed in sympathetic and parasympathetic ganglia. Fast synaptic transmission between pre- and postsynaptic neurons is achieved by neuronal nicotinic acetylcholine receptors (nAChRs). There is still little known about the subunit composition of these receptors. Establishing the subunit composition of native neuronal nAChRs is important for the understanding of their functional properties both in vivo and after expression in heterologous expression systems. We have combined in situ hybridization and autoradiography to detect the presence of mRNAs encoding subunits of neuronal nAChRs in sympathetic and parasympathetic ganglia. Inspection of the autoradiographs showed that the hybridization signal of five riboprobes (alpha3, alpha4 - 1, alpha7, beta2 and beta4) was significantly higher than the unspecific signal obtained with sense riboprobes. The distribution of alpha7 was tissue-dependent: alpha7 riboprobe binding was detected in the neurons of the superior cervical ganglion, adrenal medulla and ciliary ganglion. In contrast, the alpha7 hybridization signal was found only in a small fraction (1 - 3%) of the neurons of the sphenopalatine and otic ganglia. Our results are consistent with the idea that alpha3 mRNA expression levels are somewhat higher than those of alpha7, alpha4 - 1, beta2 and beta4.