Three-dimensional light microscopy of diploid Drosophila chromosomes.
Three-dimensional light microscopy of diploid Drosophila chromosomes.
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二倍体果蝇染色体的三维光学显微镜。
DOI：
10.1002/cm.970100106
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发表时间：
1988
影响因子：
--
通讯作者：
Sedat,JW
中科院分区：
文献类型：
--
作者：
Agard,DA;Hiraoka,Y;Sedat,JW
荧光显微镜，独特的，提供了检查完整的，甚至活的细胞内的特定成分的能力。不幸的是，高分辨率的传统荧光显微镜本质上是一种二维技术，对厚度超过0.5 μm的样品表现不佳。探索细胞内组件的空间组织需要开发针对三维数据收集、处理、显示和解释进行优化的新方法。我们对了解染色体结构和功能之间的关系的兴趣使我们开发了必要的方法来探索三维细胞结构。现在可以直接确定在整个有丝分裂细胞周期的大部分完整细胞核内二倍体染色体的三维空间组织。
Fluorescence microscopy, uniquely, provides the ability to examine specific components within intact, even living, cells. Unfortunately, high‐resolution conventional fluorescence microscopy is intrinsically a two‐dimensional technique and performs poorly with specimens thicker than about 0.5 μm. Probing the spatial organization of components within cells has required the development of new methods optimized for three‐dimensional data collection, processing, display, and interpretation. Our interest in understanding the relationship between chromosome structure and function has led us to develop the necessary methodology for exploring cell structures in three dimensions. It is now possible to determine directly the three‐dimensional spatial organization of diploid chromosomes within intact nuclei throughout most of the mitotic the cell cycle.