Identification of pterygium-related long non-coding RNAs and expression profiling by microarray analysis
Identification of pterygium-related long non-coding RNAs and expression profiling by microarray analysis
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DOI：
10.3892/ijmm.2016.2641
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发表时间：
2016-08-01
影响因子：
5.4
通讯作者：
Zhang, Xiaojun
中科院分区：
文献类型：
--
作者：
Liu, Jin;Ding, Xiangya;Zhang, Xiaojun
翼状胬肉是一种常见的眼表退行性和增殖性疾病。一旦它的长入覆盖瞳孔，它就成为一种严重威胁视力的并发症。翼状胬肉细胞的增殖能力使翼状胬肉看起来具有类似于肿瘤发生的机制。长链非编码RNA（lncRNA）是基因表达的关键调控因子。某些lncRNA的表达水平与许多疾病相关，例如不同的肿瘤和代谢紊乱等。然而，lncRNA对翼状胬肉的贡献仍然很大程度上未被探索。在这项研究中，我们构建了翼状胬肉相关的lncRNA文库，使用微阵列分析，以探讨lncRNA在翼状胬肉的发展中的潜在作用。与成对的相邻正常结膜组织相比，在翼状胬肉组织中鉴定出总共3，066个上调和1，646个下调的lncRNA（对数倍数变化>2.0）。进行定量聚合酶链反应（qPCR）以验证10名患者中3种上调和2种下调的lncRNA。生物信息学分析（Gene Ontology分析和途径分析）用于进一步的研究。通路分析表明，82条通路对应于下调的转录物，15条通路对应于上调的转录物（p值截止值，0.05）。我们的研究结果揭示了翼状胬肉中lncRNA的差异表达，提示lncRNA可能是治疗翼状胬肉的新的分子靶点。
Pterygium is a common degenerative and proliferative disease of the ocular surface. It becomes a significant sight-threatening complication once its ingrowth covers the pupil. The proliferative capacities of pterygial cells give pterygia the appearance of having a mechanism similar to tumorigenesis. Long non-coding RNAs (lncRNAs) are key regulators of gene expression. The expression levels of certain lncRNAs are associated with a number of diseases, such as different tumors and metabolic disorders, among others. However, the contributions of lncRNAs to pterygium remain largely unexplored. In this study, we constructed pterygium-related lncRNA libraries using microarray analysis to investigate the potential roles of lncRNAs in the development of pterygium. A total of 3,066 upregulated and 1,646 downregulated lncRNAs were identified in pterygium tissues compared with paired adjacent normal conjunctival tissues (log fold change >2.0). Quantitative polymerase chain reaction (qPCR) was performed to validate 3 upregulated and 2 downregulated lncRNAs in 10 patients. Bioinformatics analyses (Gene Ontology analysis and pathway analysis) were used for further research. Pathway analysis indicated that 82 pathways corresponded to the downregulated transcripts and that 15 pathways corresponded to the upregulated transcripts (p-value cut-off, 0.05). Our results reveal differentially expressed lncRNAs in pterygium and suggest that lncRNAs may be the novel molecular targets for the treatment of pterygium.