Single-Cell Acoustic Force Spectroscopy: Resolving Kinetics and Strength of T Cell Adhesion to Fibronectin
Single-Cell Acoustic Force Spectroscopy: Resolving Kinetics and Strength of T Cell Adhesion to Fibronectin
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DOI：
10.1016/j.celrep.2018.08.034
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发表时间：
2018-09-11
期刊：
影响因子：
8.8
通讯作者：
Rose, Thierry
中科院分区：
文献类型：
--
作者：
Kamsma, Douwe;Bochet, Pascal;Rose, Thierry
评估细胞与细胞外基质分子相互作用的强度和动力学是了解细胞黏附过程的基础。鉴于这些过程的相关性，迫切需要物理方法在单细胞水平上定量评估细胞黏附机制，从而能够区分具有不同行为的细胞。在这里，我们介绍单细胞声力光谱(ScAFS)，这是一种利用声波对数百个单独的细胞并行施加高达1nN的可控力的方法。我们通过测量CD4(+)T淋巴细胞(CD4)对纤维连接蛋白的粘附力和动力学来证明scAFS的潜力。我们确定，它们的主要调节细胞因子白介素7加速了CD4的黏附，而CD4的结合强度保持不变。这些细胞的激活可能会增加它们与血流中的血管壁结合的机会，从而渗透到炎症组织中，并局部协调免疫反应。
Assessing the strength and kinetics of molecular interactions of cells with the extracellular matrix is fundamental to understand cell adhesion processes. Given the relevance of these processes, there is a strong need for physical methods to quantitatively assess the mechanism of cell adhesion at the single-cell level, allowing discrimination of cells with different behaviors. Here we introduce single-cell acoustic force spectroscopy (scAFS), an approach that makes use of acoustic waves to exert controlled forces, up to 1 nN, to hundreds of individual cells in parallel. We demonstrate the potential of scAFS by measuring adhesion forces and kinetics of CD4(+) T lymphocytes (CD4) to fibronectin. We determined that CD4 adhesion is accelerated by interleukin-7, their main regulatory cytokine, whereas CD4 binding strength remains the same. Activation of these cells likely increases their chance to bind to the vessel wall in the blood flow to infiltrate inflamed tissues and locally coordinate the immune response.