Macro-SICM: A Scanning Ion Conductance Microscope for Large-Range Imaging
Macro-SICM: A Scanning Ion Conductance Microscope for Large-Range Imaging
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DOI：
10.1021/acs.analchem.7b04764
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发表时间：
2018-04-17
影响因子：
7.4
通讯作者：
Schaeffer, Tilman E.
中科院分区：
文献类型：
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作者：
Schierbaum, Nicolas;Hack, Martin;Schaeffer, Tilman E.
扫描离子电导显微镜 (SICM) 是一种多功能、高分辨率成像技术，使用充满电解质的纳米管作为探针。其非接触式成像原理使 SICM 非常适合研究液体环境中柔软而精致的表面结构。 SICM 在化学、物理和生物学领域的应用数量不断增加。然而，传统 SICM 的缺点是其扫描范围相对较小（通常横向为 100 μm X 100 μm，垂直方向为 10 μm）。我们开发了一种Macro-SICM，其横向扫描范围为25毫米×25毫米，垂直方向为0.25毫米。我们通过不同长度尺度的成像来展示 Macro-SICM 的高度多功能性：从厘米（指纹、硬币）到毫米（牛舌组织、昆虫翅膀）再到微米（细胞、延伸物）。我们应用 Macro-SICM 来研究上皮伤口愈合中的集体细胞迁移。
The scanning ion conductance microscope (SICM) is a versatile, high-resolution imaging technique that uses an electrolyte-filled nanopipet as a probe. Its noncontact imaging principle makes the SICM uniquely suited for the investigation of soft and delicate surface structures in a liquid environment. The SICM has found an ever-increasing number of applications in chemistry, physics, and biology. However, a drawback of conventional SICMs is their relatively small scan range (typically 100 mu m X 100 mu m in the lateral and 10 mu m in the vertical direction). We have developed a Macro-SICM with an exceedingly large scan range of 25 mm X 25 mm in the lateral and 0.25 mm in the vertical direction. We demonstrate the high versatility of the Macro-SICM by imaging at different length scales: from centimeters (fingerprint, coin) to millimeters (bovine tongue tissue, insect wing) to micrometers (cellular, extensions). We applied the Macro-SICM to the study of collective cell migration in epithelial wound healing.