Imperatorin Ameliorates the Aging-Associated Porcine Oocyte Meiotic Spindle Defects by Reducing Oxidative Stress and Protecting Mitochondrial Function.
Imperatorin Ameliorates the Aging-Associated Porcine Oocyte Meiotic Spindle Defects by Reducing Oxidative Stress and Protecting Mitochondrial Function.
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DOI：
10.3389/fcell.2020.592433
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发表时间：
2020
影响因子：
5.5
通讯作者：
Kim NH
中科院分区：
文献类型：
--
作者：
Luo D;Zhang JB;Li SP;Liu W;Yao XR;Guo H;Jin ZL;Jin YX;Yuan B;Jiang H;Kim NH
欧前胡素具有抗氧化、抗炎、抗菌、抗癌、抗高血压等多种药理活性。然而，其对动物生殖系统，特别是卵母细胞发育、成熟和衰老的影响尚不清楚。本研究旨在探讨IMP对卵母细胞发育和衰老的影响及其分子机制。将卵母细胞再培养24小时用于老化。结果表明，IMP（40 μM）处理能显著提高孤雌激活的老化卵母细胞的囊胚形成率和孵化率。同时，IMP处理后，观察到分布均匀的皮质颗粒，但透明质酸硬度无显著差异。在此阶段，细胞内活性氧，细胞凋亡和自噬水平下降，而线粒体膜电位，谷胱甘肽水平，超氧化物歧化酶和过氧化氢酶的活性增加。IMP处理的老化卵母细胞也表现出显着更高的表达MOS，CCNB1，BMP 15，和GDF 9比非IMP处理的老化卵母细胞，但它们的水平仍然低于新鲜卵母细胞。以上结果表明，IMP可以通过降低氧化应激和保护线粒体功能，有效改善猪卵母细胞的老化质量。
Imperatorin (IMP) exhibits a variety of pharmacological properties, including antioxidant, anti-inflammatory, antibacterial, anti-cancer, and anti-hypertension activities. However, its effects on animal reproduction systems, especially oocyte development, maturation, and aging are not yet clear. In this study, the effects of IMP on oocyte development and aging as well as the underlying molecular mechanisms were explored. Oocytes were cultured for an additional 24 h for aging. Results revealed that the blastocyst formation and hatching rates of embryos, which were parthenogenetically activated aged oocytes, were significantly increased with IMP treatment (40 μM). Simultaneously, well-distributed cortical granules but no significant difference in zona pellucida hardness were observed after IMP treatment. During this stage, intracellular reactive oxygen species, apoptosis, and autophagy levels were decreased, while mitochondrial membrane potential, glutathione level, and activity of superoxide dismutase and catalase were increased. IMP-treated aged oocytes also showed significantly higher expression of MOS, CCNB1, BMP15, and GDF9 than non-IMP-treated aged oocytes although their levels were still lower than those in the fresh oocytes. These results suggest that IMP can effectively ameliorate the quality of aged porcine oocytes by reducing oxidative stress and protecting mitochondrial function.