Comparative transcriptomics reveals key differences in the response to milk oligosaccharides of infant gut-associated bifidobacteria.
Comparative transcriptomics reveals key differences in the response to milk oligosaccharides of infant gut-associated bifidobacteria.
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DOI：
10.1038/srep13517
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发表时间：
2015-09-04
影响因子：
4.6
通讯作者：
Mills DA
中科院分区：
文献类型：
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作者：
Garrido D;Ruiz-Moyano S;Lemay DG;Sela DA;German JB;Mills DA
母乳增强了婴儿肠道中双歧杆菌属的优势，这可能是由于母乳中含有大量的人乳低聚糖（HMO）。在这里，我们筛选婴儿肠道分离的长双歧杆菌亚种。使用单独的HMO，比较了主要HMO上代表性分离株的全局转录组。而B. HMO利用模式一致，B。两歧的菌株更多样化，一些菌株不使用岩藻糖基乳糖（FL）或唾液酸乳糖（SL）。B的转录组两歧SC 555和B. ATCC 15697显示，混合HMO的利用与中性HMO相似，而在FL上生长的转录组比B中的HMO更类似于乳糖。两歧与HMO利用相关的基因被中性HMO和SL上调，但在两个物种中不被FL上调。相反，FL诱导了B中替代基因簇的表达。一群人。结果还表明，B.双歧杆菌SC 555不利用来自HMO的岩藻糖或唾液酸。令人惊讶的是，即使在相同的基质上生长，两种双歧杆菌之间的直链淀粉基因的表达也不同。这项研究强调了在双歧杆菌属中发现的两种主要策略来处理HMO，并提供了关于HMO和婴儿肠道双歧杆菌之间密切关系的详细信息。
Breast milk enhances the predominance of Bifidobacterium species in the infant gut, probably due to its large concentration of human milk oligosaccharides (HMO). Here we screened infant-gut isolates of Bifidobacterium longum subsp. infantis and Bifidobacterium bifidum using individual HMO, and compared the global transcriptomes of representative isolates on major HMO by RNA-seq. While B. infantis displayed homogeneous HMO-utilization patterns, B. bifidum were more diverse and some strains did not use fucosyllactose (FL) or sialyllactose (SL). Transcriptomes of B. bifidum SC555 and B. infantis ATCC 15697 showed that utilization of pooled HMO is similar to neutral HMO, while transcriptomes for growth on FL were more similar to lactose than HMO in B. bifidum. Genes linked to HMO-utilization were upregulated by neutral HMO and SL, but not by FL in both species. In contrast, FL induced the expression of alternative gene clusters in B. infantis. Results also suggest that B. bifidum SC555 does not utilize fucose or sialic acid from HMO. Surprisingly, expression of orthologous genes differed between both bifidobacteria even when grown on identical substrates. This study highlights two major strategies found in Bifidobacterium species to process HMO, and presents detailed information on the close relationship between HMO and infant-gut bifidobacteria.