P-cadherin and E-cadherin are co-expressed in MDCK cells.
P-cadherin and E-cadherin are co-expressed in MDCK cells.
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P-钙粘蛋白和E-钙粘蛋白在MDCK细胞中共表达。
DOI：
10.1006/bbrc.1993.2189
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发表时间：
1993
影响因子：
3.1
通讯作者：
DePhilip,RM
中科院分区：
文献类型：
--
作者：
Wu,JC;Gregory,CW;DePhilip,RM
Madin-Darby 犬肾 (MDCK) 细胞系是上皮细胞功能的重要模型，E（上皮）-钙粘蛋白在 MDCK 细胞活性中的作用已得到充分认识。这里使用 Northern 和免疫印迹分析来证明，除了 E-钙粘蛋白之外，MDCK 细胞培养物还表达 P（胎盘）-钙粘蛋白。然后使用免疫细胞化学来比较 P-和 E-钙粘蛋白的定位和去垢剂可提取性。虽然两种钙粘蛋白都在细胞与细胞接触的区域发现，但提取 P-钙粘蛋白的相对容易性表明，与 E-钙粘蛋白相比，其对肌动蛋白细胞骨架的锚定较弱，并且可能与 E-钙粘蛋白相比，细胞与细胞之间的相互作用不太持久。 MDCK 细胞对不同钙粘蛋白的共表达尚未得到充分探索，可能会影响以前仅考虑 E-钙粘蛋白活性的 MDCK 细胞功能的解释。
The Madin-Darby canine kidney (MDCK) cell line is a valuable model of epithelial cell function and the role of E(epithelial)-cadherin in MDCK cell activity is well-appreciated. Northern and immunoblot analyses were used here to demonstrate that, in addition to E-cadherin, MDCK cell cultures also express P(placental)-cadherin. Immunocytochemistry was then used to compare the localization and detergent extractability of P- and E-cadherin. While both cadherins were found at areas of cell-cell contact, the relative ease with which P-cadherin could be extracted suggests a weaker anchorage to the actin cytoskeleton than has been demonstrated for E-cadherin, and may correspond to less permanent cell-cell interactions than those attributed to E-cadherin. The co-expression of different cadherins by MDCK cells has not been fully explored and may influence interpretations of MDCK cell function that previously considered only the activity of E-cadherin.