Modulation of mice fecal microbiota by administration of casein glycomacropeptide
Modulation of mice fecal microbiota by administration of casein glycomacropeptide
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酪蛋白糖巨肽对小鼠粪便微生物群的调节
DOI：
10.4081/mr.2012.e3
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发表时间：
2012-01-01
影响因子：
1.5
通讯作者：
Pang, Guangchang
中科院分区：
文献类型：
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作者：
Chen, Qingsen;Cao, Jinyi;Pang, Guangchang
酪蛋白糖巨肽 (GMP) 已知可促进双歧杆菌和乳酸杆菌的体外生长。在本文中，我们采用传统培养技术和荧光原位杂交（FISH）技术来研究酪蛋白GMP对小鼠粪便微生物群的影响。测试并比较了肠道微生物群的种群结构，包括乳杆菌、双歧杆菌、肠球菌、大肠菌群和肠杆菌科。连续给予酪蛋白GMP 15 d后，乳酸菌和双歧杆菌数量显着增加（P<0.01），肠杆菌和大肠菌数量显着下降（P<0.05），而肠球菌无明显变化。 FISH技术的检出限显着低于传统培养方法（P<0.01）。这些结果表明，摄入酪蛋白 GMP 对雄性 BALB/c 小鼠具有益生元作用。酪蛋白 GMP 有助于建立更健康的肠道微生物群。此外，FISH被证明是一种快速且相对低成本的检测方法，可用于进一步了解人类肠道微生物群。
Casein glycomacropeptide (GMP) is known to promote the in vitro growth of Bifidobacteria and Lactobacilli . In this paper, we used conven- tional culture techniques and fluorescent in situ hybridization (FISH) techniques to investigate the effect of casein GMP on mice fecal microbiota. The population structure of the intestinal microbiota, including Lactobacillus , Bifidobacteria , Enterococcus , coliforms and Enterobacteriaceae , was tested and compared. After consecutive administration of casein GMP for 15 days, numbers of Lactobacillus and Bifidobacteria increased significantly (P<0.01), numbers of Enterobacteriaceae and Coliforms decreased significantly (P<0.05) while no significant changes were observed for Enterococcus. The detection limits of FISH technique were significantly lower (P<0.01) than the traditional culture method. These results suggested that consumption of casein GMP had a prebiotic effect on male BALB/c mice. Casein GMP helped establish a healthier intestinal microbiota. Additionally, FISH was proved to be a rapid and relatively low-cost detection method that can be used to further our understanding of human intestinal microbiota.