Peroxisome biogenesis in the yeast Hansenula polymorpha is controlled by a complex set of interacting gene products.
Peroxisome biogenesis in the yeast Hansenula polymorpha is controlled by a complex set of interacting gene products.
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多形汉逊酵母中的过氧化物酶体生物发生是由一组复杂的相互作用的基因产物控制的。
DOI：
10.1073/pnas.90.16.7470
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发表时间：
1993
影响因子：
11.1
通讯作者：
Veenhuis,M
中科院分区：
文献类型：
--
作者：
Titorenko,VI;Waterham,HR;Cregg,JM;Harder,W;Veenhuis,M
我们研究了酵母中过氧化物酶体生物合成所必需的12个基因（命名为PER 1-PER 12）中突变等位基因之间的遗传相互作用。这些基因中的任何一个的隐性突变确定了三种不同的形态表型：（i）完全不存在过氧化物酶体（Per-）;（ii）存在小过氧化物酶体与胞质溶胶中的过氧化物酶体基质蛋白的主要部分（Pim-）;和（iii）存在具有异常结晶基质亚结构的过氧化物酶体（Pss-）。广泛的互补分析表明，许多情况下的noncomplementation-也就是说，二倍体包含两个不同的PER基因的野生型和突变体等位基因不能生长在甲醇和过氧化物酶体缺陷。观察到的非连锁非互补的情况下，似乎是基因和等位基因特异性，主要是在较低的温度下观察到（冷敏感）。所获得的遗传结果被用来制定PER基因产物相互作用的模型。在这个模型中，五个PER基因产物是复合物的关键或核心组分。其他PER基因产物似乎发挥更外围的作用。
We have studied the genetic interactions between mutant alleles in 12 genes, designated PER1-PER12, which are essential for peroxisome biogenesis in the yeast Hansenula polymorpha. Recessive mutations in any of these genes determined three different morphological phenotypes: (i) complete absence of peroxisomes (Per-); (ii) presence of small peroxisomes in conjunction with a major fraction of peroxisomal matrix proteins in the cytosol (Pim-); and (iii) presence of peroxisomes with aberrant crystalline matrix substructure (Pss-). Extensive complementation analysis showed many cases of noncomplementation--that is, diploids that contained both wild-type and mutant alleles of two different PER genes were unable to grow on methanol and showed peroxisomal defects. The observed cases of unlinked noncomplementation appeared to be gene and allele specific and were predominantly observed at lower temperatures (cold sensitive). The genetic results obtained were used to formulate a model of PER gene product interactions. In this model, five PER gene products are key or core components of the complex. Other PER gene products appear to play a more peripheral role.