The stability of markers in dried-blood spots for recommended newborn screening disorders in the United States
The stability of markers in dried-blood spots for recommended newborn screening disorders in the United States
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DOI：
10.1016/j.clinbiochem.2011.09.010
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发表时间：
2011-12-01
影响因子：
2.8
通讯作者：
Hannon, W. H.
中科院分区：
文献类型：
--
作者：
Adam, B. W.;Hall, E. M.;Hannon, W. H.
目的：我们旨在分别测量热量和湿度对干血斑（DBS）样本中34种先天性疾病标志物水平变化的贡献。设计和方法：我们将配对的DBSs在低湿度和高湿度环境中以预定的间隔在37℃下保存。在单个分析运行中测量每个完整样本集中所有样本的标记水平。结果:研究30 + / - 5天,期间galactose-1-phosphate uridyltransferase biotinidase失去了几乎65%的初始活动在低湿度存储:大多数其他27个标记的退化是由于副作用的高湿存储:7个标记dbs储存在高湿度损失了超过90%的初始水平的研究和4 7跌逾50%的初始水平的第一周内存储。结论：降低DES运输和储存环境中的湿度和温度对保持样品完整性至关重要。(C) 2011加拿大临床化学学会。Elsevier Inc.出版。版权所有。
Objective: We aimed to measure separately the contributions of heat and humidity to changes in levels of 34 markers of inborn disorders in dried-blood-spot (DBS) samples.Design and methods: We stored paired sets of DBSs at 37 degrees C for predetermined intervals in low-humidity and high-humidity environments. Marker levels of all samples in each complete sample set were measured in a single analytic run.Results: During the 30 +/- 5 day studies, galactose-1-phosphate uridyltransferase and biotinidase lost almost 65% of initial activities in low-humidity storage: most of the degradation in 27 other markers was attributable to adverse effects of high-humidity storage: seven markers in DBSs stored at high humidity lost more than 90% of initial levels by the end of the study and 4 of the 7 lost more than 50% of initial levels within the first week of storage.Conclusions: Minimizing both humidity and temperature in DES transportation and storage environments is essential to maintaining sample integrity. (C) 2011 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.