Effects of CrylAb toxin on Propylea japonica (Thunberg) (Coleoptera: Coccinellidae) through its prey, Nilaparvata lugens St(a)over-circlel (Homoptera: Delphacidae), feeding on Transgenic Bt rice
Effects of CrylAb toxin on Propylea japonica (Thunberg) (Coleoptera: Coccinellidae) through its prey, Nilaparvata lugens St(a)over-circlel (Homoptera: Delphacidae), feeding on Transgenic Bt rice
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DOI：
10.1603/0046-225x-35.4.1130
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发表时间：
2006-08-01
影响因子：
1.7
通讯作者：
Wang, D.
中科院分区：
文献类型：
--
作者：
Bai, Y. Y.;Jiang, M. X.;Wang, D.
采用含苏云金芽孢杆菌（Bacillus thuringiensis Berliner, Bt） cry1Ab合成基因的水稻进行室内取食实验，研究Bt水稻取食猎物对日本螟（Propylea japonica, Thunberg）的影响。以表达Bt-cr-y1Ab的2个纯合子转基因水稻品系克明道1号（KMD1）和克明道2号（KMD2）及其未转化亲本品种秀水11号（XS11）为材料，获得植株。试验的草食性猎物为褐飞虱（Nilaparvata lugens Stal），是水稻最严重的害虫之一，KMD1和KMD2均未针对褐飞虱。采用酶免疫吸附法测定了Cry1Ab毒素在KMD1和KMD2植株上的表达浓度以及转移到取食这些植株上的N. lugens的浓度。用KMD1-和kmd2 -饲养的粳稻发育参数进行了室内评价。结果表明，水稻叶片和茎中Cry1Ab浓度在孕穗期至灌浆期显著升高，随后随植株成熟而降低。在以Bt水稻为食的褐飞虱若虫和成虫体内均可检测到Cry1Ab。以KMD1-和kmd2 -为食的蠋蝽幼虫的发育时间、化蛹率、成虫羽化率、蛹和成虫体重、雄-成虫运动活性与以xs11为食的蠋蝽幼虫无显著差异。总之，我们的研究结果表明，非目标昆虫N. lugens及其捕食者P. japonica暴露于转基因Cry1Ab水稻的Cry1Ab毒素，但该捕食者的发育不受毒素的三营养相互作用的影响。
Laboratory feeding experiments using rice plants containing a synthetic cry1Ab gene derived from Bacillus thuringiensis Berliner (Bt) were carried out to study the effects of Bt rice-fed prey on the predator Propylea japonica (Thunberg). Plants were obtained from two homozygous transgenic Bt-cr-y1Ab expressing rice lines, Kemingdao 1 (KMD1) and Kemingdao 2 (KMD2), and their untransformed parental variety Xiushui 11 (XS11). The herbivorous prey species tested was the brown planthopper, Nilaparvata lugens Stal, one of most serious insect pests of rice and not targeted by KMD1 or KMD2. The concentrations of Cry1Ab toxin expressed in KMD1 and KMD2 plants and that of the toxin transferred to N. lugens feeding on these plants were determined by enzyme immunosorbent assay technique. Development parameters of F. japonica reared on KMD1- or KMD2-fed N. lugens were assessed in the laboratory. The results showed that the concentration of Cry1Ab in rice leaves and stems significantly increased from the booting to grain filling stage and subsequently decreased as the plants matured. Cry1Ab could be detected in nymphs and adults of N. lugens feeding on the Bt rice plants. Development time, pupation, adult eclosion, pupal and adult weight, and male-adult locomotive activity of P. japonica that had preyed on KMD1- or KMD2-fed N. lugens nymphs as larvae were not significantly different from those that preyed on XS11-fed nymphs. In short, our results indicate that the nontarget insect N. lugens and its predator P. japonica are exposed to Cry1Ab toxin from transgenic cry1Ab rice, but development of this predator was not affected by the toxin through tritrophic interactions.