Evaluation of VEGF-A in platelet and microRNA-126 in serum after coronary artery bypass grafting
Evaluation of VEGF-A in platelet and microRNA-126 in serum after coronary artery bypass grafting
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DOI：
10.1007/s00380-021-01855-6
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发表时间：
2021-04-20
影响因子：
1.5
通讯作者：
Imoto, Yutaka
中科院分区：
文献类型：
--
作者：
Mukaihara, Kosuke;Yamakuchi, Munekazu;Imoto, Yutaka
血小板功能被认为有助于心脏手术后的临床结果。本研究旨在评估血管内皮生长因子-A（VEGF-A）和microRNA-126（miR-126）在冠状动脉旁路移植术（CABG）中的关键作用。从2013年7月至2014年3月期间接受CABG手术的67例患者中采集全血用于血小板分离。在不同时间点测量血清、血浆和血小板中的VEGF-A和miR-126水平，并与临床特征进行比较。术后3天血小板计数下降。常规冠状动脉搭桥术（CCAB）后血小板计数的动态变化大于非体外循环冠状动脉搭桥术（OPCAB）。CABG术后第3天相同数量血小板中的VEGF-A（IP-VEGF-A）升高，术后第7天血清中的VEGF-A（S-VEGF-A）升高。血清中miR-126- 3 p水平（S-miR-126- 3 p）在CABG后迅速升高，然后降至术前水平以下。CABG术后第7天，外周动脉疾病（PAD）患者中存在内皮功能障碍的IP-VEGF-A水平高于无PAD患者。相反，术后第7天，PAD患者的S-miR-126- 3 p低于无PAD患者。血管内皮功能障碍导致的S-miR-126- 3 p水平降低可能导致IP-VEGF-A水平升高，IP-VEGF-A水平升高与CABG术后并发症密切相关。
Platelet functions are thought to contribute to clinical outcomes after heart surgery. This study was conducted to assess the pivotal roles of vascular endothelial growth factor-A (VEGF-A) and microRNA-126 (miR-126) during coronary artery bypass grafting (CABG). Whole blood was collected for platelet isolation from 67 patients who underwent CABG surgery between July 2013 and March 2014. VEGF-A and miR-126 levels in serum, plasma, and platelets were measured at various time points and compared with clinical characteristics. The platelet count was decreased at 3 days after CABG. This dynamic change in platelet count was larger after conventional coronary artery bypass (CCAB) than off-pump coronary artery bypass (OPCAB). VEGF-A in the same number of platelets (IP-VEGF-A) was increased at 3 days after CABG, followed by an increase of VEGF-A in serum (S-VEGF-A) at 7 days after surgery. The miR-126-3p level in serum (S-miR-126-3p) increased rapidly after CABG and then decreased below preoperative levels. The IP-VEGF-A level on day 7 after CABG in patients with peripheral artery disease (PAD), who suffered from endothelial dysfunction, was higher compared with patients without PAD. Conversely, S-miR-126-3p on day 7 after surgery was lower in patients with PAD than in patients without PAD. Low levels of S-miR-126-3p due to endothelial dysfunction may lead to high IP-VEGF-A, which is closely related to complications after CABG.