Comparative Proteomics Reveals the Mechanisms Underlying Variations in Seed Vigor Based on Maize (Zea mays L.) Ear Positions
Comparative Proteomics Reveals the Mechanisms Underlying Variations in Seed Vigor Based on Maize (Zea mays L.) Ear Positions
复制标题
比较蛋白质组学揭示了基于玉米 (Zea mays L.) 穗位的种子活力变化的机制
DOI：
10.1007/s11105-018-1115-x
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发表时间：
2018-12-01
影响因子：
2.1
通讯作者：
Zhang, Chunqing
中科院分区：
文献类型：
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作者：
Li, Yan;Qu, Haibin;Zhang, Chunqing
种子活力受种子在植物体内的位置影响。然而，目前对其潜在机制的了解有限。本研究采用等压标记相对和绝对定量技术，研究了玉米(Zea Mays L.)中粒(活力较高)和顶粒(Zea Mays L.)的比较蛋白质组。吸胀0小时、24小时、48小时的耳数。共鉴定出159个差异积累的蛋白质。其中，来自疾病/防御和新陈代谢功能类别的蛋白质数量最多。与顶端种子相比，中部种子在吸胀0h和24h时蛋白质合成和能量的差异积累较多，而48h吸胀时积累较少。种子吸收水分会激活新陈代谢过程。在吸胀12~30h之间，中层种子的水分含量显著低于顶层种子，但在吸胀24 h时，中层种子的产能能力高于顶层种子。同时，液泡膜固有蛋白3.1和3.2在吸胀24 h时在中部种子中表现出较高的积累量。液泡膜固有蛋白3.1和3.2是介导水分流入蛋白质贮藏液泡，进而激活参与储备动员的酶的。此外，我们的数据还显示，中间种子遭受的真菌损害可能较小。我们的结果有助于理解生长位置对种子活力影响的潜在机制。
Seed vigor is influenced by seed position in plant. However, current understanding of its underlying mechanism is limited. In this study, we used isobaric tags for relative and absolute quantitation technique to study the comparative proteomes between middle seeds (with higher vigor) and top seeds of maize (Zea mays L.) ears at 0 h, 24 h, and 48 h of imbibition. A total of 159 differentially accumulated proteins were identified. Among these, the largest number of proteins was from the functional categories of Disease/Defense and Metabolism. Compared with top seeds, most of the differentially accumulated proteins of Protein Synthesis and Energy showed higher accumulation in middle seeds at 0 h and 24 h of imbibition, but lower accumulation at 48 h of imbibition. Seed water absorption activates metabolic processes. The water content of middle seeds was significantly lower than that of top seeds at between 12 h and 30 h of imbibition, but energy production would be higher in the middle seeds at 24 h of imbibition. Meanwhile, tonoplast intrinsic proteins 3.1 and 3.2, which mediate water inflow into protein storage vacuoles, then activating enzymes involved in reserve mobilization, showed higher accumulation in middle seeds at 24 h of imbibition. In addition, our data also showed middle seeds may suffer less fungal damages. Our results contribute to understanding the mechanisms underlying the effects of growth position on seed vigor.