Cylindrospermopsin induces neurotoxicity in tilapia fish (Oreochromis niloticus) exposed to Aphanizomenon ovalisporum
Cylindrospermopsin induces neurotoxicity in tilapia fish (Oreochromis niloticus) exposed to Aphanizomenon ovalisporum
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DOI：
10.1016/j.aquatox.2015.01.024
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发表时间：
2015-04-01
影响因子：
4.5
通讯作者：
Camean, Ana M.
中科院分区：
文献类型：
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作者：
Guzman-Guillen, Remedios;Lomares Manzano, Inmaculada;Camean, Ana M.
Cyn是几种淡水蓝藻产生的一种细胞毒性氰基毒素，如卵孢囊藻等。半胱氨酸是一种三环生物碱，具有抑制蛋白质和谷胱甘肽合成的能力，并能改变哺乳动物和脊椎动物中不同的氧化应激生物标志物。虽然肝脏和肾脏似乎是这种毒素的主要Cyn目标，但它也会影响其他器官。在鱼类中，目前还没有证据表明半胱氨酸的神经毒性。本研究旨在研究半胱氨酸氨基转移酶(Cyn)对尼罗罗非鱼(Oreochromis Niloticus)脑内乙酰胆碱酯酶(AChE)活性、脂质过氧化(LPO)水平和组织病理学的影响。结果表明，亚慢性暴露于毒素的尼罗罗非鱼(O.niloticus)脑组织中AChE活性显著抑制，LPO水平升高，并出现相应的组织病理学变化。此外，我们还对这些参数的潜在恢复进行了研究，在清洁的未受污染的水中经历了两个委托期(3天和7天)，表明从净化3天起生化参数就恢复了，而恢复组织病理学变化需要7天。为了支持这些结果，用酶联免疫吸附试验(ELISA)检测了所有暴露鱼脑中的半胱氨酸氨基转移酶(Cyn)，并观察了净化时间的影响。基于这些结果，首次证实了半胱氨酸的神经毒性及其在亚慢性暴露的罗非鱼脑组织中的存在。(C)2015爱思唯尔B.V.保留所有权利。
Cylindrospermopsin (CYN) is a cytotoxic cyanotoxin produced by several species of freshwater cyanobacteria, such as Aphanizomenon ovalisporum. CYN is a tricyclic alkaloid known for its ability to inhibit both protein and glutathione synthesis, and the alteration of different oxidative stress biomarkers in mammals and vertebrates. Although the liver and kidney appear to be the main CYN targets for this toxin, it also affects other organs. In fish, there is no evidence about the neurotoxicity of CYN yet. In the present study, we aimed to study the potential neurotoxicity of CYN, based on the measure of Acetylcholinesterase (AChE) activity, lipid peroxidation (LPO) levels and histopathological studies in brain of tilapia (Oreochromis niloticus) subchronically exposed to repeated concentrations of 10 mu g CYN/L by immersion in an A. ovalisporum culture for 14 days. The results showed significant inhibition of AChE activity and increases in LPO levels, as well as relevant histopathological alterations in the brain of fish (O. niloticus) subchronically exposed to the toxin. Moreover, we also investigated the potential recovery of these parameters by subjecting the fish to two deputation periods (3 and 7 days) in clean uncontaminated water, showing a recovery of the biochemical parameters since 3 days of depuration, and being necessary 7 days to recover the histopathological changes. In order to support these results, CYN was detected and quantified by enzyme-linked immunosorbent assay (ELISA) in brain of all the exposed fish and the effects of the depuration periods were also observed. Based on these results, it was demonstrated for the first time the neurotoxicity of CYN and its presence in brain of tilapia fish subchronically exposed to CYN. (C) 2015 Elsevier B.V. All rights reserved.