INHIBITION OF THYMIDYLATE BIOSYNTHESIS INDUCES MITOTIC UNEQUAL SISTER CHROMATID RECOMBINATION IN SACCHAROMYCES-CEREVISIAE
INHIBITION OF THYMIDYLATE BIOSYNTHESIS INDUCES MITOTIC UNEQUAL SISTER CHROMATID RECOMBINATION IN SACCHAROMYCES-CEREVISIAE
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DOI：
10.1007/bf00417818
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发表时间：
1984-01-01
期刊：
影响因子：
2.5
通讯作者：
HAYNES, RH
中科院分区：
文献类型：
--
作者：
KUNZ, BA;TAYLOR, GR;HAYNES, RH
抑制DTMP生物合成导致酿酒酵母单倍体菌株核糖体DNA基因簇中LEU2插入缺失。插入片段的丢失通过增加扇区(Leu+/Leu-)和非扇区(Leu-)菌落的产生来检测。对Leu+和Leu-区DNA进行Southern印迹分析，得到的杂交图谱与姐妹染色单体不等重组合的发生情况相一致。RAD6基因的缺陷并不能阻止RAD52-1突变对克隆的诱导。在RAD52-1和RAD6株中，胸腺嘧啶核苷耗竭均可诱导形成非分段的白细胞集落。
Inhibition of dTMP biosynthesis induced deletion of a LEU2 insert from the ribosomal DNA gene cluster of haploid strains of S. charomyces cerevisiae. Loss of the insert was detected phenotypically by the enhanced production of both sectored (leu+/leu-) and non-sectored (leu-) colonies. Hybridization patterns obtained by Southern blot analysis of DNA from the leu+ and leu- sectors were consisted with the occurrence of unequal sister chromatid recombination. The induction of sectored colonies was prevented by the rad52-1 mutation but not by defects in RAD6. The formation of non-sectored leu- colonies was induced by thymidylate depletion in both rad52-1 and rad6 strains.