Alternate poliovirus nonstructural protein processing cascades generated by primary sites of 3C proteinase cleavage.
Alternate poliovirus nonstructural protein processing cascades generated by primary sites of 3C proteinase cleavage.
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DOI：
10.1016/0042-6822(92)90193-s
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发表时间：
1992-11
期刊：
影响因子：
3.7
通讯作者：
M. Lawson;B. Semler
中科院分区：
文献类型：
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作者：
M. Lawson;B. Semler
小核糖核酸病毒基因表达的翻译后调控介导的级联加工的病毒蛋白质还没有得到很好的理解。感染细胞的脉冲追踪研究和从基因组cDNA克隆转录的脊髓灰质炎病毒1型RNA翻译的体外研究都表明了多蛋白加工的特定级联反应，其中P1、P2和P3前体蛋白是病毒蛋白酶切割的主要产物。我们报告的结果，脊髓灰质炎病毒1型蛋白加工在anin vitrotranslation系统和在感染的HeLa细胞，这表明存在另一个，快速途径的3C蛋白酶的活性介导的多蛋白加工的短时间动力学分析。所观察到的途径不同于先前已知的途径，并且是先前已知的途径的补充。这种替代加工途径在病毒基因表达的翻译后调节中的潜在作用进行了讨论。
The post-translational regulation of picornavirus gene expression mediated by the cascade processing of viral proteins is not well understood. Both pulse-chase studies of infected cells andin vitrostudies of the translation of poliovirus type 1 RNA transcribed from genomic cDNA clones indicate a specific cascade of polyprotein processing in which the P1, P2, and P3 precursor proteins are primary products of viral proteinase cleavage. We report the results of a short-time kinetic analysis of poliovirus type 1 protein processing in anin vitrotranslation system and in infected HeLa cells which indicate the existence of another, rapid pathway of polyprotein processing mediated by the activity of the 3C proteinase. The observed pathway is distinct from and in addition to the one previously known. The potential role of this alternative pathway of processing in the post-translational regulation of viral gene expression is discussed.