Hydrogen sulfide promotes proliferation and neuronal differentiation of neural stem cells and protects hypoxia-induced decrease in hippocampal neurogenesis
Hydrogen sulfide promotes proliferation and neuronal differentiation of neural stem cells and protects hypoxia-induced decrease in hippocampal neurogenesis
复制标题
硫化氢促进神经干细胞增殖和神经元分化，并保护缺氧引起的海马神经发生减少
DOI：
10.1016/j.pbb.2013.11.009
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发表时间：
2014-01-01
影响因子：
3.6
通讯作者：
Hao, Aijun
中科院分区：
文献类型：
--
作者：
Liu, Dexiang;Wang, Zhen;Hao, Aijun
越来越多的证据表明，硫化氢（H2S）作为一种新的神经调节剂和神经保护剂，但它仍有待研究是否H2S对神经干细胞（NSCs）的直接影响。在本研究中，我们研究了H2S供体，硫氢化钠（NaHS）对小鼠神经干细胞和海马神经发生的影响。我们在这里报告，NaHS促进神经干细胞的增殖和神经元分化。进一步的分析表明，NaHS诱导的增殖与细胞外信号调节激酶（ERK）1/2的磷酸化有关，神经元分化与分化相关基因的表达改变有关。另外，用NaHS处理缺氧的C57 BL/6小鼠（1日龄），以探讨H2S是否会影响海马神经发生。BrdU掺入实验结果表明，NaHS可增加缺氧小鼠海马齿状回增殖细胞数。此外，Morris水迷宫实验表明，NaHS治疗改善了小鼠缺氧后的认知障碍。这些发现表明，H2S可能提供一种新的治疗策略来干预脑部疾病的进展。(C)2013 Elsevier Inc. All rights reserved.
Accumulating evidence has suggested that hydrogen sulfide (H2S) acts as a novel neuro-modulator and neuroprotective agent; however, it remains to be investigated whether H2S has a direct effect on neural stem cells (NSCs). In the present study, we examined the effects of H2S donor, sodium hydrosulfide (NaHS) on mouse NSCs and hippocampal neurogenesis. We report here that NaHS promoted proliferation and neuronal differentiation of NSCs. Further analysis revealed that NaHS-induced proliferation was associated with phosphorylation of extracellular signal-regulated kinase (ERK) 1/2 and neuronal differentiation was linked to altered expression of differentiation-related genes. In addition, C57BL/6 mice (1 day old) subjected to hypoxia were treated with NaHS to explore whether H2S would influence the neurogenesis of hippocampus. BrdU incorporation assay results showed that administration of NaHS could increase the number of proliferating cells in the dentate gyrus of hippocampus in the mice after hypoxia. Moreover, Morris water maze test showed that treatment with NaHS improved cognitive impairment after hypoxia in mice. These findings suggest that H2S may afford a novel therapeutic strategy to intervene in the progression of brain diseases. (C) 2013 Elsevier Inc. All rights reserved.