CHARACTERIZATION OF 2 DIFFERENT GENES (CDNA) FOR CYTOCHROME-C OXIDASE SUBUNIT VLA FROM HEART AND LIVER OF THE RAT
CHARACTERIZATION OF 2 DIFFERENT GENES (CDNA) FOR CYTOCHROME-C OXIDASE SUBUNIT VLA FROM HEART AND LIVER OF THE RAT
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DOI：
10.1002/j.1460-2075.1988.tb03083.x
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发表时间：
1988-08-01
期刊：
影响因子：
11.4
通讯作者：
KADENBACH, B
中科院分区：
文献类型：
--
作者：
SCHLERF, A;DROSTE, M;KADENBACH, B
通过对大鼠肝脏和大鼠心脏的cDNA库进行抗体筛选，获得了两个大鼠细胞色素c氧化酶肝脏和心脏特异亚基的克隆。在心脏cDNA序列中，在第一个蛋氨酸密码子的上游18bp5‘’处发现了一个TAA终止密码子，因此排除了该蛋白的前导序列。这两个cDNA包含两个亚基的全长编码区。两个亚基的氨基酸序列仅有50%的同源性，而大鼠心脏和牛心VIA的氨基酸序列有74%的同源性。Northern印迹分析表明，来自心脏的VIA亚基基因只在心脏和骨骼肌中表达，而来自肝脏的VIA亚基基因也在肾脏、脑、心脏表达，在肌肉中表达很弱。
By antibody screening of a rat liver and a rat heart cDNA library in .lambda.gt11 two clones coding for the liver- and heart-specific subunit VIa of rat cytochrome c oxidase were isolated. In the heart cDNA sequence a TAA stop codon was found in frame 18 bp 5'' upstream of the first methionine codon, thus excluding a leader sequence for this protein. The two cDNAs contain the full-length coding region of two subunits. The amino acid sequences of the two subunits show only 50% homology, whereas 74% homology was found between rat heart and bovine heart subunit VIa. By Northern blot analysis it is shown that the gene for subunit VIa from heart is only expressed in heart and skeletal muscle, whereas that from liver is also expressed in kidney, brain, heart and weakly in muscle.