REGULATION OF THE ACTIVITY OF A NEW INHIBITOR OF ANGIOGENESIS BY A CANCER SUPPRESSOR GENE
REGULATION OF THE ACTIVITY OF A NEW INHIBITOR OF ANGIOGENESIS BY A CANCER SUPPRESSOR GENE
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DOI：
10.1016/0092-8674(89)90238-9
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发表时间：
1989-02-10
期刊：
影响因子：
64.5
通讯作者：
BOUCK, NP
中科院分区：
文献类型：
--
作者：
RASTINEJAD, F;POLVERINI, PJ;BOUCK, NP
已在仓鼠细胞和仓鼠-人杂交细胞的条件培养基中鉴定出抑制大鼠角膜中体内新生血管形成的抑制剂。抑制活性与转化体和回复体、分离杂交体和温度限制转化体中活性抑癌基因的存在紧密相关。它与一个. apprx共同纯化。140 kD糖蛋白。多克隆抗血清对纯化的制剂认识到一个140 kd的蛋白质在条件培养基中，并能够吸附出所有的抗血管生成活性。这些结果定义了作为在BHK 21/c113细胞中有活性的癌症抑制基因的一个功能的新血管形成抑制剂的活性的控制，并且同时鉴定了对实体瘤生长至关重要的过程血管生成的新抑制剂。
An inhibitor has been identified in the conditioned medium of hamster cells and hamster-human hybrids that suppresses neovascularization in vivo in the rat cornea. Inhibitory activity was tightly linked to the presence of an active cancer suppressor gene in transformants and revertants, in segregating hybrids, and in temperature-limited transformants. It copurified with a .apprx. 140 kd glycoprotein. Polyclonal antiserum raised against the purified preparation recognized a 140 kd protein in conditioned medium and was able to adsorb out all antiangiogenic activity. These results define the control of the activity of an inhibitor of neovascularization as one function of the cancer suppressor gene active in BHK21/c113 cells and simultaneously identify a new inhibitor of angiogenesis, a process vital to the growth of solid tumors.