Reduction of concanavalin A-induced expression of interferon-γ by bovine lactoferrin in feline peripheral blood mononuclear cells
Reduction of concanavalin A-induced expression of interferon-γ by bovine lactoferrin in feline peripheral blood mononuclear cells
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DOI：
10.1016/j.vetimm.2004.12.016
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发表时间：
2005-05-01
影响因子：
1.8
通讯作者：
Naito, Y
中科院分区：
文献类型：
--
作者：
Kobayashi, S;Sato, R;Naito, Y
乳酚宁（LF）是一种存在于乳汁、粘膜分泌物和中性粒细胞中的糖蛋白，有助于宿主防御和免疫调节。在本研究中，我们研究了牛LF（bLF）对伴刀豆球蛋白A（ConA）刺激的猫外周血单个核细胞（PBMC）中细胞因子信使RNA（mRNA）表达的影响。半定量逆转录聚合酶链反应（RT-PCR）和实时荧光PCR显示，在猫PBMC中，ConA诱导的干扰素-γ（IFN-γ）mRNA表达增加，但白细胞介素-1 β（IL-1 β），肿瘤坏死因子-α（TNF-α）和IL-12 p40 mRNA的表达没有增加。这种ConA诱导的IFN-γ mRNA表达的增加不仅在ConA刺激前30 min，而且在ConA刺激后10、20和40 min被加入bLF抑制。Western blotting结果显示，猫PBMC中的蛋白酪氨酸激酶（PTK）和细胞外信号调节激酶（ERK）在ConA刺激后10 min内被激活，刺激后40 min这两种激酶的激活几乎消失。此外，ConA诱导的IFN-γ mRNA的表达部分地被Genistein（一种全局性PTK抑制剂）和PD-98059（一种ERK抑制剂）分别阻止。这些结果表明，bLF是能够抑制刀豆蛋白A诱导的IFN-γ mRNA的表达，废除后激活刀豆蛋白A和它的受体之间的相互作用的细胞内信号。(c)2004 Elsevier B. V.保留所有权利。
Lactofenin (LF), a glycoprotein present in milk, mucosal secretions and neutrophils, contributes to host defense and immunomodulation. In the present study, we investigated the effect of bovine LF (bLF) on cytokine messenger RNA (mRNA) expression in concanavalin A (ConA)-stimulated feline peripheral blood mononuclear cells (PBMC). Semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) and real-time PCR showed a ConA-induced increase of interferon-gamma (IFN-gamma) mRNA expression but not of interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha) and IL-12 p40 mRNA in feline PBMC. This ConA-induced increase of IFN-gamma mRNA expression was inhibited by addition of bLF not only 30 min before ConA stimulation but also 10, 20 and 40 min after ConA stimulation. Western blotting showed that protein tyrosine kinase (PTK) and extracellular signal-regulated kinase (ERK) in feline PBMC were activated within 10 min after the ConA stimulation and that the activation of both kinases had almost disappeared by 40 min after stimulation. Moreover, the ConA-induced IFN-gamma mRNA expression was partly prevented by genistein, a global PTK inhibitor, and PD-98059, an ERK inhibitor, respectively. These results suggest that bLF is able to inhibit the ConA-induced IFN-gamma mRNA expression by abrogation of intracellular signaling activated after interaction between ConA and its receptor. (c) 2004 Elsevier B.V. All rights reserved.