Catecholamine biosynthetic enzymes are expressed in replicating cells of the peripheral but not the central nervous system.
Catecholamine biosynthetic enzymes are expressed in replicating cells of the peripheral but not the central nervous system.
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儿茶酚胺生物合成酶在外周神经系统的复制细胞中表达，但在中枢神经系统中不表达。
DOI：
10.1073/pnas.77.10.6221
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发表时间：
1980
影响因子：
11.1
通讯作者：
Reis,DJ
中科院分区：
文献类型：
--
作者：
Rothman,TP;Specht,LA;Gershon,MD;Joh,TH;Teitelman,G;Pickel,VM;Reis,DJ
我们试图确定鸡和大鼠发育中的外周和中枢神经系统中含儿茶酚胺神经元的前体在其退出细胞周期之前是否表达生物合成酶酪氨酸羟化酶[THase;酪氨酸3-单加氧酶; L-酪氨酸，四氢蝶啶：氧氧化还原酶（3-羟基化），EC 1.14.16.2]或多巴胺β-羟化酶[DBHase; 3，4-二羟基苯乙胺，抗坏血酸盐：氧氧化还原酶（β-羟基化），EC 1.14.17.1]。鸡胚（26-27期）注射[~ 3 H-胸腺嘧啶核苷，4小时后制备放射自显影标记的免疫反应THase细胞核。对暴露于[3 H]胸苷2小时的大鼠胎仔（E12-E14天）的脑和交感神经链进行类似处理，不同之处在于外周组织用DBHase特异性抗体和抗THase染色。在鸡和大鼠的周围神经系统中，放射自显影标记含TH酶的细胞核。大鼠外周神经系统中含DBHase的细胞也被标记，因此是去甲肾上腺素能细胞。THase定位于相同大鼠胎仔脑细胞中，从E12天开始（E11或E11.5天未检测到THase），位于腹侧中脑和头外侧菱形细胞群的外套层中;然而。中枢神经系统中含TH酶的细胞未掺入[3 H]胸苷。我们的结论是，在发展过程中，肾上腺素能神经元前体的周围神经系统，但不是中央，有能力合成儿茶酚胺之前，他们退出细胞周期。外周和中枢神经元成熟的差异可能与其胚胎起源的差异有关。
We sought to determine whether the precursors of catecholamine-containing neurons in the developing peripheral and central nervous systems of chickens and rats express the biosynthetic enzymes tyrosine hydroxylase [THase; tyrosine 3-monooxygenase; L-tyrosine, tetrahydropteridine: oxygen oxidoreductase (3-hydroxylating), EC 1.14.16.2] or dopamine beta-hydroxylase [DBHase; 3,4-dihydroxyphenylethylamine, ascorbate:oxygen oxidoreductase (beta-hydroxylating), EC 1.14.17.1], prior to the time they withdraw from the cell cycle. Chicken embryos (stages 26-27) were injected with [3H-thymidine and 4 hr later were prepared for the simultaneous demonstration of radioautographically labeled nuclei in immunoreactive THase cells. The brains and sympathetic chains of rat fetuses (days E12-E14), exposed for 2 hr to [3H]thymidine, were treated similarly except that peripheral tissues were stained with a specific antibody to DBHase as well as anti-THase. In the peripheral nervous system of both chicken and rat, nuclei of THase-containing cells were radioautographically labeled. DBHase-containing cells in the peripheral nervous system of rats were also labeled and thus are noradrenergic. THase was localized in cells of the brain of the same rat fetuses beginning on day E12 (no THase was detected on day E11 or E11.5) in the mantle layer of the ventral mesencephalic and rostrolateral rhombocephalic cellular groups; however. THase-containing cells in the central nervous system did not incorporate [3H]thymidine. We conclude that, during development, the adrenergic neuronal precursors of the peripheral nervous system but not of the central, have the capacity to synthesize catecholamines before they withdraw from the cell cycle. Differences in the maturation of peripheral and central neurons may be related to differences in their embryological origin.