Regeneration of plants from callus tissues of hairy roots induced by Agrobacterium rhizogenes on Alhagi pseudoalhagi.
Regeneration of plants from callus tissues of hairy roots induced by Agrobacterium rhizogenes on Alhagi pseudoalhagi.
复制标题
DOI：
--
复制
发表时间：
2001
期刊：
影响因子：
44.1
通讯作者：
Y. Wang;J. Wang;D. Luo;J. Jia
中科院分区：
文献类型：
--
作者：
Y. Wang;J. Wang;D. Luo;J. Jia
以豆科牧草骆驼刺的子叶和下胚轴切段为侵染材料，用发根农杆菌A4菌株转化骆驼刺。从发生在感染部位或附近的毛状根产生的无菌愈伤组织获得再生植株。毛状根再生植株的叶片形态和茎生长正常，但根系较浅，比正常植株更发达。Opine合成、PCR和Southern杂交证实T-DNA已整合到A.拟骆驼刺基因组乙酰丁香酮（AS）是成功转化A.假骆驼刺
The legume forage Alhagi pseudoalhagi was transformed by the Agrobacterium rhizogenes strain A4 using cotyledon and hypocotyl segments as infection materials. Regenerated plants were achieved from sterile calli derived from hairy roots, which occurred at or near the infection sites. The regenerated plants from hairy root were characterized by normal leaf morphology and stem growth but a shallow and more extensive root system than normal plants. Opine synthesis, PCR and Southern blot confirmed that T-DNA had been integrated into the A. pseudoalhagi genome. Acetosyringone (AS) was found to be vital for successful transformation of A. pseudoalhagi.