HPLC of basic drugs using non-aqueous ionic eluents: evaluation of a 3 mum strong cation-exchange material.
HPLC of basic drugs using non-aqueous ionic eluents: evaluation of a 3 mum strong cation-exchange material.
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DOI：
10.1002/bmc.1292
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发表时间：
2009-07
期刊：
影响因子：
--
通讯作者：
P. Morgan;V. Manwaring;R. Flanagan
中科院分区：
文献类型：
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作者：
P. Morgan;V. Manwaring;R. Flanagan
在使用含有离子改性剂（如高氯酸铵）的100%甲醇洗脱液分析碱性药物时，填充有3 μ m粒度HPLC Technology Techsphere SCX（丙磺酸改性）二氧化硅的HPLC色谱柱比5 μ m SCX填料具有相当大的优势。研究的基本药物包括氯氮平和去甲氯氮平、奥氮平、奎宁和奎尼丁、阿米替林、去甲替林、丙咪嗪和地昔帕明。与5微米材料相比，3微米柱不仅对于给定的柱长更有效，而且洗脱时间更短，这是在反相系统中观察到的现象。用3微米SCX改性填料获得的高效率和优异的峰形以及获得的相对低的背压表明，这些材料值得实验室在分析基本药物时认真考虑。制造商应提供这种包装作为一种常规。如果需要，可使用替代的离子改性剂，如乙酸铵，用于质谱检测。
HPLC columns packed with 3 microm particle size HPLC Technology Techsphere SCX (propylsulfonic acid-modified) silica offer considerable advantages over 5 microm SCX packings in the analysis of basic drugs using 100% methanol eluents containing an ionic modifier such as ammonium perchlorate. The basic drugs studied included clozapine and norclozapine, olanzapine, quinine and quinidine, and amitriptyline, nortriptyline, imipramine and desipramine. The 3 microm column was not only more efficient for a given column length compared with 5 microm materials, but also elution times were less, a phenomenon observed in reversed-phase systems. The high efficiencies and excellent peak shapes obtained with the 3 microm SCX-modified packing together with the relatively low back-pressures attained show that such materials deserve serious consideration by laboratories involved in the analysis of basic drugs. Manufacturers should offer such packings as a matter of routine. Alternative ionic modifiers such as ammonium acetate are available for use with mass spectrometric detection if required.