CD38 expression on CD8+ T lymphocytes as a marker of residual virus replication in chronically HIV-infected patients receiving antiretroviral therapy
CD38 expression on CD8+ T lymphocytes as a marker of residual virus replication in chronically HIV-infected patients receiving antiretroviral therapy
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DOI：
10.1089/088922204773004950
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发表时间：
2004-02-01
影响因子：
1.5
通讯作者：
Soriano, V
中科院分区：
文献类型：
--
作者：
Benito, JM;López, M;Soriano, V
血液中CD8(+)CD38(+) T淋巴细胞水平与hiv感染者的疾病进展相关，独立于CD4计数。尽管CD8（+）细胞上的CD38表达在大多数受试者中并没有完全正常化，但有效的抗逆转录病毒治疗可以在血浆病毒血症的同时减少这一淋巴细胞亚群，这可能反映了残留的HIV复制。用流式细胞术定量测定了200个个体中CD38在CD8（+）细胞上的表达（每个CD8（+）细胞中CD38分子的数量），其中170个是HIV阳性，30个是HIV未感染的对照组。46名艾滋病毒感染者正在接受抗逆转录病毒治疗，病毒载量无法检测到。其余124名艾滋病毒阳性患者未接受治疗，并有可检测到的血浆病毒血症。hiv阳性、未经治疗的患者CD8（+）细胞上CD38的平均水平高于接受抗病毒治疗的患者和对照组（每个CD8（+）细胞分别为5023、2029和1978分子，p < 0.01）。在hiv阳性、未经治疗的受试者中，CD38的高表达主要发生在CD45RO（+）和CD8（+）细胞上。CD38水平与血浆HIV-RNA呈正相关（r = 0.63,p < 0.001）。在hiv阳性受试者开始抗逆转录病毒治疗后，CD8（+）细胞上的CD38水平稳步下降。少数个体出现病毒突变，而在抗病毒治疗中，CD8（+）细胞上的CD38水平随着病毒复制的发作而短暂增加。在慢性HIV感染中，CD8（+）细胞上的CD38水平升高，并且与血浆病毒血症密切相关。在接受抗逆转录病毒治疗的血浆病毒血症患者中，CD8（+）细胞上CD38表达随着时间的推移而缓慢下降，这表明CD8（+）细胞上CD38表达可以用作残留病毒复制的标记。
The level of CD8(+)CD38(+) T lymphocytes in blood correlates with disease progression in HIV-infected individuals, independently of the CD4 count. Effective antiretroviral therapy reduces this lymphocyte subset in parallel with plasma viremia, although CD38 expression on CD8(+) cells does not normalize completely in most subjects, and might reflect residual HIV replication. The expression of CD38 on CD8(+) cells (as number of CD38 molecules per CD8(+) cell) was measured quantitatively by flow cytometry in 200 individuals, of whom 170 were HIV positive and 30 were HIV-uninfected controls. Forty-six HIV-infected subjects were on antiretroviral therapy and had undetectable viral load. The remaining 124 HIV-positive persons were not on therapy and had detectable plasma viremia. The mean level of CD38 on CD8(+) cells was higher in HIV-positive, untreated patients than in subjects on antiviral therapy and controls (5023, 2029, and 1978 molecules per CD8(+) cell, respectively, p < 0.01). In HIV-positive, untreated subjects, the higher CD38 expression mainly occurred on CD45RO(+)CD8(+) cells. The level of CD38 strongly correlated with plasma HIV-RNA (r = 0.63,p < 0.001). The levels of CD38 on CD8(+) cells declined steadily in HIV-positive subjects after beginning antiretroviral therapy. A few individuals presented viral blips whereas being on antiviral treatment, levels of CD38 on CD8(+) cells increased transiently in parallel with episodes of viral replication. Levels of CD38 on CD8(+) cells are increased in chronic HIV infection, and strongly correlate with plasma viremia. The slow decline of CD38 expression on CD8(+) cells over time in subjects with undetectable plasma viremia while being on antiretroviral therapy suggests that CD38 expression on CD8(+) cells could be used as a marker of residual virus replication.