Differential effect of thermal stress on HSP70 expression, nitric oxide production and cell proliferation among native and crossbred dairy cattle
Differential effect of thermal stress on HSP70 expression, nitric oxide production and cell proliferation among native and crossbred dairy cattle
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DOI：
10.1016/j.jtherbio.2016.04.012
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发表时间：
2016-07-01
影响因子：
2.7
通讯作者：
Deb, Rajib
中科院分区：
文献类型：
--
作者：
Bhanuprakash, V.;Singh, Umesh;Deb, Rajib
在印度这样的热带国家，热应激是显著影响奶牛生产力的主要因素之一。本研究的目的是确定在印度的Sahiwal和荷斯坦杂交（Frieswal）人口的细胞活力，有丝分裂原刺激指数，一氧化氮的产生和热休克蛋白70表达的热和冷应激的影响。结果表明，由于Sahiwal牛外周血单个核细胞（PBMC）和植物血凝素（PHA-P）促分裂原刺激指数的存活率较高，Sahiwal牛能显著（P < 0.05）地抵抗冷热应激的影响。这项研究还揭示了Sahiwal和Frieswal杂交样品的热应激前后样品中的一氧化氮（μ M）产生水平显著降低（P < 0.05）。热休克蛋白70的表达明显高于对照组，（P < 0.05）加热后立即在Sahiwal中高于Frieswal/间接ELISA分析显示，热应激和冷应激（0 h）后，Hsp 70蛋白浓度（ng/ml）立即逐渐升高，并在恢复6 h时达到峰值。Western blot和免疫荧光检测结果也与间接ELISA结果相一致。夏峰期（44 ℃）和冬峰期（10 ℃），萨希瓦尔牛HSP 70 mRNA表达量和蛋白质含量均显著高于弗里斯瓦尔牛（P < 0.05）。这项调查支持了早期的信息更高的适应性土著牛品种的炎热和潮湿的条件下相比，温带牛品种的杂交。(C)2016爱思唯尔有限公司版权所有
In a tropical country like India, thermal stress is one of the major factors which significantly affects the productivity of dairy cattle. The present study was aimed to identify the effect of heat and cold stress on cell viability, mitogen stimulation indices, nitric oxide production and HSP70 expression in Sahiwal and Holstein crossbred (Frieswal) population in India. The results indicated that the Sahiwal breed can better withstand the effect of heat and cold stress significantly (P < 0.05) when compared to the crossbred cattle due to the higher survivability of the Peripheral Blood Mononuclear Cells (PBMCs) and Phytohemagglutinin (PHA-P) mitogen based stimulation indices. The study also revealed the significant differences (P < 0.05) in the level of nitric oxide (mu M) production amongst the pre and post thermal stressed samples of Sahiwal and Frieswal crossbred samples.Further, the expression of HSP70 was significantly (P < 0.05) higher in Sahiwal compared to Frieswal immediately after heat/cold shock to 6 h of recovery as indirect ELISA analysis showed gradual rise in the Hsp70 protein concentration (ng/ml) immediately after heat and cold stress (0 h) and reached the peak at 6 h of recovery. Western blot and immune fluorescent assay results were also corroborated with the findings of indirect ELISA. In Sahiwal cattle the mRNA expression of HSP70 and its protein concentration were higher (P < 0.05) during peak summer (44 degrees C) and winter (10 degrees C) as compared to Frieswal cattle. This investigation supports the earlier information on the higher adaptability of indigenous cattle breeds to hot and humid conditions compared to the crossbreds of temperate cattle breeds. (C) 2016 Elsevier Ltd. All rights reserved.